Vicki Doronina

Vicki did her PhD in Molecular Biology at the University of Edinburgh. She had been working as a postdoc in several Russel group UK universities, while honing her skills in scientific and creative writing. She is now a pen for hire. Check out my proudest achievement, which may be useful for you: The BiteSizeBio Guide for Protein expression

Articles by Vicki Doronina:

Assembling the Puzzle: Cloning with Compatible Cohesive Ends

Consider a jigsaw puzzle. While most of the pieces have a different picture on their surface, all pieces fit together in an interlocking pattern. As unlikely as it may seem, restriction enzymes from different organisms can produce interlocking pieces of DNA – so called compatible cohesive ends (CCE). These are pieces of DNA, which fit…

17 Oct 2014 DNA / RNA Manipulation and Analysis

Look Ma, No Kit!: A DIY Method for Isolating Yeast Genomic DNA

I recently moved to a different research institute and was happy to discover that my new lab had not one but several different kits for yeast genomic DNA isolation. I like trying new kits and protocols. I especially like trying new kits when they promise, like the Olympics, to yield results that are “Swifter, Higher,…

08 Oct 2014 DNA / RNA Manipulation and Analysis

A Sneak Peak at ‘The Bitesize Bio Guide to Protein Expression’ – a Bitesize Bio eBook

If I piqued your interest in the first post about my new e-Book ‘The Bitesize Bio Guide to Protein Expression – a Bitesize Bio eBook’ check out this excerpt from the book explaining what an expression system is and how to choose the right one.  What is an expression system anyway? There was a time…

23 Sep 2014 Protein Expression & Analysis

Have I Got Good Protein Expression News for You

While the art of reading hand poured sequencing gels is forgotten as the next generation sequencers spit out genome after genome, protein expression still often requires sweat, tears and a lot of luck. I think this happens not because there is something different about protein expression in comparison with sequencing, but because people do not…

21 Sep 2014 Protein Expression & Analysis

Stand By Your Poster

A lot of scientists hate presenting posters. Because more often than not, you stand near your carefully designed, full of great results placard, all alone, while there is a crowd gathered two posters down. There are some conclusions you could come to: You might think that the popular poster author’s work is brilliant – unlike…

08 Sep 2014 Writing, Publishing & Presenting

Dpn I The Strange

If you ever used a site-directed mutagenesis kit or ligation-independent cloning, then you also used restriction enzyme Dpn I. But what does it do and more interestingly, why? Restriction enzymes and methylases: the yin and yang of bacteria Usual restriction enzymes, the toolkit of genetic engineering, are one half of the “yin and yang” pair…

15 Aug 2014 DNA / RNA Manipulation and Analysis

Seven Frivolous Reasons Not to Attend a Conference

Scientific conferences are a peculiar throwback to XIX century, when bearded white men in wool suits were meeting in ale houses to discuss the latest experiment they did at home – once. In our time of instant communication and telepresence, conferences are obsolete and I’ll tell you why. 1. The main reason not to go…

13 Aug 2014 Fun Stuff

Kick Start Your Research With Crowdfunding!

Like most scientists, you rely on grant monies to fund your research. Sustaining it depends on your ability to devise promising new ideas, collect new data and show proof-of-concept before writing another proposal to keep the cycle going. Only the best-of-the-best pilot projects seem to make the cut for experimentation when scarce lab funds are…

06 Aug 2014 Inspiring & Thought Provoking

Shooting Trouble During Cloning

As frustration goes, cloning is often up there with trying to thread a camel through the eye of a needle. You do everything carefully: prepare your vector and fragment DNA, cut them as the restriction enzyme manufacturer instructs (complete digest in five minutes!), ligate, transform and go home in anticipation of a good number of…

04 Jul 2014 DNA / RNA Manipulation and Analysis

Tips for Heating up Agar in the Microwave

One of our readers posted the following question to us and we decided to pass it along to everybody’s favorite microbiology expert, Aunt Yersinia: For one year I am working in different research laboratories, after I got from school. I keep wondering why EVERYBODY is using pre-made Agar solutions for pouring plates, and EVERYBODY is…

17 Feb 2014 Basic Lab Skills & Know-how