Cynthia Barber's Profile

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The Many Flavors of Widefield Microscopy

While confocal microscopy seems to have become pervasive in cell biology, widefield microscopy techniques still have a special and important place. This month on the Microscopy and Imaging Channel, we’re focusing on widefield microscopy techniques: covering the basics of what these techniques are and when you should turn to them. What is this ‘Widefield’ you […]

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In Microscopy & Imaging 11th of February, 2014
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That Other Number – The Meaning Of Numerical Aperture In Microscopy

Perhaps while staring at your microscope during long imaging sessions, you have noticed that written on your objective lens is not only the magnification, but also another number such as: NA=1.4. What does that number mean anyway? Meet the numerical aperture (NA for short)! Not a number for the casual user While the numerical aperture […]

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In Microscopy & Imaging 23rd of July, 2013
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How To Name Image Files So They Actually Make Sense – The Good, The Bad And The Ugly

I have a dear friend and collaborator whose image file names follow this format: aaaa5kk.tif, aaaa5kkk.tif, aaaa5kkkk.tif, aaaa6p3kkkkkkk.tif, etc. We have collaborated together on several projects, and I dread the days I have to go back through the files and look for a particular image. I am sure that these names have some meaning to […]

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In Microscopy & Imaging 14th of May, 2013
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STORM, PALM And fPALM- The Alphabet Soup Of Super-Resolution Light Microscopy

Why do I see a cloud? When you are looking at protein localization within a cell, have you ever wondered why you see a cloud of fluorescence rather that several individual fluorescent points? Well, light microscopy has a theoretical resolution limit of 200 nm. This means that in theory, to resolve two points as being […]

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In Microscopy & Imaging 19th of March, 2013