SKennedy

Suzanne is Director of R&D at Mo Bio Laboratories in California, and the author of their blog, The Culture Dish. She has a PhD in Microbiology and Immunology from Virginia Commonwealth University.

Articles by SKennedy:

When Glycogen is not Your Friend – Isolating RNA from Glycogen-Rich Tissues

Bitesize Bio has had a lot to say about RNA isolation, mainly because it is one of the most anxiety-producing requirements for molecular biology; especially when you are first starting out (although isolating proteins from complex samples like soil and stool is far more difficult, let me tell you.  But that’s a future post.)  We’ve…

16 Apr 2014 DNA / RNA Manipulation and Analysis

Troubleshooting RNA Isolation

Isolating RNA is one of the more finicky protocols there is, and everyone who does it has their own personal tips and tricks to successfully isolate intact RNA from their samples with consistency. Although RNA can be somewhat unpredictable since it is so labile, there are a few common problems that occur which can be logically…

19 Mar 2014 DNA / RNA Manipulation and Analysis

10 Signs You’ll Like Working in Biotech

The decision to make the switch from an academic lab and career to a biotech company doesn’t come easy. Many scientists are wary of the lack of independence and doing science for profit.  But there are advantages too. Working in a biotech lab allows you to work on many varied and interesting projects that actually come to…

11 Aug 2010 Career Development & Networking

Important Considerations for a Career in Biotech

When I was in graduate school, it seemed that almost no one aspired to work in industry or be part of a company. But times are changing. Now, when I go to conferences and talk to scientists in training, I am asked, “how do I get a job in a company?” and “How did you get…

29 Jul 2010 Career Development & Networking

What it Takes to Take the Lead

I had the unique opportunity to attend a lecture by the CEO of Life Technologies, Greg Lucier, just recently here in San Diego. The lecture was organized by Leo Lee, the creator of a new group on Linkedin.com called Leadership Builder and the title of the presentation was “Developing Executive Leadership: What it Takes to…

06 Jul 2010 Personal Development

How DNA extraction kits work in the lab

At Bitesize Bio, we share a lot of troubleshooting tips for RNA and genomic DNA extraction because almost everything we do in molecular biology requires nucleic acid isolation as the very first step. These days, most labs use commercial DNA extraction kits based on spin column technology. Spin columns contain a silica resin that selectively…

28 Jun 2010 DNA / RNA Manipulation and Analysis

Kids Read Science Summer Reading Contest 2010

Joanne Manaster is a woman on a mission. She loves science and she wants to introduce kids to everything there is to love about science. So this summer, her mission is to stimulate the minds of children and teens everywhere by challenging them to read non-fiction science books. In collaboration with Jeff Shaumeyer, program director of  www.scienticity.net, she has organized a…

09 Jun 2010 Fun Stuff

MIQE Guidelines: Do Your RT-qPCRs Make The Grade?

Northern and Southern blotting are now a thing of the past. They’ve been replaced with a faster and more quantitative technique. No longer do we wait days to know whether a gene is expressed. We can have the answer in 45 minutes! Real-time PCR is now commonly employed in almost all molecular biology laboratories to…

04 Jun 2010 PCR, qPCR and qRT-PCR

Do Hand Sanitizer and Liquid Hand Soap Remove Viruses?

While reading my back issues of Applied and Environmental Microbiology (AEM), I came across an interesting paper that detailed an in-depth study on the effectiveness of hand cleaners to remove Norwalk virus (NV) from intentionally contaminated hands. Yes that’s right – intentionally contaminated, and how. The study volunteers allowed a 20% stool suspension containing Norwalk virus to be…

26 May 2010 Lab Safety

Important Considerations for Determining qPCR Efficiency

One of the very first things you need to do when getting set up for quantitative PCR (qPCR) is to determine the efficiency of the assay because knowing the assay efficiency is critical to accurate data interpretation. And you have to do this every time you design and purchase a new primer pair. Ideally, the…

19 Apr 2010 PCR, qPCR and qRT-PCR