SKennedy

Suzanne is Director of R&D at Mo Bio Laboratories in California, and the author of their blog, The Culture Dish. She has a PhD in Microbiology and Immunology from Virginia Commonwealth University.

Articles by SKennedy:

No More White Elephants! – Consider this Before Buying a Real-time PCR Cycler

Does your lab have a closet full of white elephants; once expensive instruments that are no longer fit for purpose, or have broken down? In many cases, all of that wasted money and resource could have been saved if the buyers had made smart choices about matching the instrument more closely to their needs. A…

09 Jul 2016 PCR, qPCR and qRT-PCR

10 Tips for Consistent Real-Time PCR

Real-time PCR is a specialized technique that delivers far more information about your DNA or RNA than end-point PCR. Essentially, real-time PCR is a way to visualize the amplification of specific DNA fragments as it is happening (in real time) and allows for the ability to quantify exactly how much DNA (or RNA) was in…

09 Jul 2016 PCR, qPCR and qRT-PCR

There’s No Need To Be Paranoid About RNA Purification

RNA purification may be a common procedure in molecular biology but it is by far the one that people fear most. Why? Dreaded RNase. It’s everywhere… all over your bench and pipettors, and floating in the air, waiting for the chance to creep into your prep, shred your RNA into nucleotides, and ruin a day’s…

09 Jul 2016 DNA / RNA Manipulation and Analysis

10 Tips For Better DNA Gel Extraction Results

What is it about DNA gel extraction that makes it a pain? Maybe it’s poor product yields or maybe it’s because the process uses harsh chemicals (chaotropic salts, ethidium bromide, ethanol, heat) that will damage or denature DNA and potentially decrease cloning success. In this article I share some tips, both from experience and from…

09 Jul 2016 DNA / RNA Manipulation and Analysis

The Key to Unlocking DNA from FFPE Tissues

Formalin fixed paraffin embedded (FFPE) tissues are valuable samples that typically come from human specimens collected for examination of the histology of biopsies for the detection of cancer. But each sample contains much more information just waiting to be unlocked. Despite the tiny sample size, DNA can be extracted from the tissue sections and used…

09 Jul 2016 Microscopy & Imaging

How DNA Extraction Kits Work in the Lab

We give a lot of troubleshooting help on RNA and DNA extraction here at Bitesize Bio because almost everything we do in molecular biology requires DNA or RNA at the very first step.  These days, most labs use commercial DNA extraction kits, which employ spin columns, for the isolation of DNA and RNA. The spin columns…

28 Jul 2015 DNA / RNA Manipulation and Analysis

DNA Precipitation: Ethanol vs. Isopropanol

Since our most popular article of all time (“The Basics: How Ethanol Precipitation of DNA and RNA Works”) was published, many of our readers have asked us to further explain the difference between precipitating DNA with ethanol vs. isopropanol and which is the better choice. So today, I’ll meet the challenge and discuss the pros…

23 Jun 2015 DNA / RNA Manipulation and Analysis

5 Types of Bad Boss and How to Handle Them

Science attracts so many different and quirky personalities that you are bound to have a problem with some people. But when your boss is the problem, its a big problem for you. So what do you do when you don’t get along with your boss? Well sometimes the best advice is really to just move…

10 Dec 2014 Dealing with Fellow Scientists

Plasmid vs. Genomic DNA Extraction: The Difference

To isolate plasmid DNA, you crack your cells open and perform a miniprep, trying hard to avoid contaminating genomic DNA. For genomic DNA, you crack your cells open in a different way and try to isolate as much of the contents as possible. So what’s the difference in the protocols? In this article, we will…

08 Oct 2014 DNA / RNA Manipulation and Analysis

Better Plasmid Midipreps Part II: What Causes Low Yields?

Recently we received a question from Bitesize Bio reader Sonia after our article How to: Get Better Plasmid Midiprep Yields. She asked: “What could be the problem when one sample gives a good yield while the other plasmid gives poor a one, when both the samples were processed simultaneously, and in the same way.” This is a…

12 May 2014 DNA / RNA Manipulation and Analysis