Olwen Reina

I am a Clinical Research Coordinator at the U.S. Department of Veterans Affairs with a background in basic research, writing, mentoring and teaching. I studied Natural Science at Trinity College Dublin, Ireland, specializing in biochemistry with immunology and I am currently undergoing ACRP (Association of Clinical Research Professionals) certification. In my spare time, I enjoy studying HTML/CSS and SEO, doing acroyoga, making kombucha, salsa dancing, voluntary community projects and eating sushi. Feel free to send me a note with any writing opportunities or to say hello.

Articles by Olwen Reina:

Time to Instigate Nested PCR

How to Obtain a Purer PCR Product and Reduce Non-specific Amplification Unless you’ve gotten your hands on some miraculously specific primers, amplification of only your target sequence without non-specific amplification can be very challenging. Thankfully, a clever and surprisingly simple solution is at hand! A Quick Recap of the Basics In PCR, you design your…

09 Jul 2016 PCR, qPCR and qRT-PCR

Comparing Viral Vector Expression Systems

Some viral vectors are the little black dresses of cloning and expression experiments: They work for almost any occasion and always give you the results you were hoping for. Other vectors are more like ballgowns that only come out of storage for special occasions. Let’s wade through all the information out there and take a…

09 Jul 2016 DNA / RNA Manipulation and Analysis

Native Versus Denaturing Gels

We’re already gone through the basics of how gel electrophoresis work, compared common gel types like agarose and polyacrylamide and even explored some alternatives. Now let’s look at the native versus denaturing gels. You’ll be a speGEList in no time! Denaturing Gels We’ll start with this one, as it’s very self-explanatory. Denaturing gels are exactly…

09 Jul 2016 Protein Expression & Analysis

The BOOM Method for Nucleic Acid Purification: The Ultimate Chick Flick?

The Boom method, or Boom nucleic acid extraction method, is a solid phase extraction technique for isolating nucleic acids from a solution of biological matter. This is just a fancy way of saying you use this technique to expose and remove the nucleic acids from a cell. First developed by William R. Boom, the Boom…

09 Jul 2016 DNA / RNA Manipulation and Analysis

Protein Extraction and Solubilization using the TRIZOL® Method

Extracting protein from tissue samples and cultured cells is Step #1 in many biochemical and analytical techniques. Before you can do a Polyacrylamide Gel Electrophoresis (PAGE), a Western blot, or mass spectrometry you need to extract your protein. Nowadays, a lot of labs have switched to kits for their protein extraction but these kits can…

09 Jul 2016 Protein Expression & Analysis

Expanding Possibilities: Why You Need to Look into Viral Transduction

We have already looked into the different types of viral expression systems and when you might use one over another in my previous article. But why would you use viral transduction over similar techniques like plasmids? Just a reminder: Transfection is a lab technique where nucleic acids or proteins may be introduced into cells. When…

09 Jul 2016 Cells and Model Organisms

How to Clean and Maintain Scales

Spring time means spring cleaning! Baby birds are hatching, the days are getting longer, there are butterflies everywhere and you feel inspired to do a good deep clean of your lab scales. We can’t help you sing like Snow White so that animals come help you clean but we can help you get your scales…

22 Jun 2015 Basic Lab Skills & Know-how

Get To Know Your DNA Polymerases

While most may think standard Taq is the backbone of PCR, there are many other DNA polymerase options out there. The polymerase you use has a significant impact on the efficacy of your PCR, specifically on the product yield, the purity of the product and the faithfulness with which the starting product is transcribed. Sometimes,…

18 Jun 2015 PCR, qPCR and qRT-PCR

Fuzzy Wuzzy Problems: Achieving and Maintaining a Contamination-free Incubator

When you share an incubator with a number of people it can be very hard to keep a clean shop and months, or more, of work can be lost due to contamination. The two biggest sources of bugs in an incubator are: the water pan the containers being put in and out. Both of these…

17 Jun 2015 Cells and Model Organisms

Problems amplifying GC-rich regions? Problem Solved!

No, it isn’t you that’s the problem, and you’re certainly not alone if you’re having trouble amplifying GC-rich sequence and/or understanding why GC-rich sequences are causing such problems in the first place! Amplification of GC-rich sequences by PCR has been an irritant for scientists for decades! When we say “GC-rich” we mean ?60% of the…

26 May 2015 Lab Statistics & Math