Olwen Reina's Profile

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Comparing Viral Vector Expression Systems

Some viral vectors are the little black dresses of cloning and expression experiments: They work for almost any occasion and always give you the results you were hoping for. Other vectors are more like ballgowns that only come out of storage for special occasions. Let’s wade through all the information out there and take a […]

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Expanding Possibilities: Why You Need to Look into Viral Transduction

We have already looked into the different types of viral expression systems and when you might use one over another in my previous article. But why would you use viral transduction over similar techniques like plasmids? Just a reminder: Transfection is a lab technique where nucleic acids or proteins may be introduced into cells. When […]

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In Cells and Model Organisms 9th of July, 2016
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Native Versus Denaturing Gels

We’re already gone through the basics of how gel electrophoresis work, compared common gel types like agarose and polyacrylamide and even explored some alternatives. Now let’s look at the native versus denaturing gels. You’ll be a speGEList in no time! Denaturing Gels We’ll start with this one, as it’s very self-explanatory. Denaturing gels are exactly […]

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In Protein Expression & Analysis 9th of July, 2016
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The BOOM Method for Nucleic Acid Purification: The Ultimate Chick Flick?

The Boom method, or Boom nucleic acid extraction method, is a solid phase extraction technique for isolating nucleic acids from a solution of biological matter. This is just a fancy way of saying you use this technique to expose and remove the nucleic acids from a cell. First developed by William R. Boom, the Boom […]

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Protein Extraction and Solubilization using the TRIZOL® Method

Extracting protein from tissue samples and cultured cells is Step #1 in many biochemical and analytical techniques. Before you can do a Polyacrylamide Gel Electrophoresis (PAGE), a Western blot, or mass spectrometry you need to extract your protein. Nowadays, a lot of labs have switched to kits for their protein extraction but these kits can […]

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In Protein Expression & Analysis 9th of July, 2016
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How to Clean and Maintain Scales

Spring time means spring cleaning! Baby birds are hatching, the days are getting longer, there are butterflies everywhere and you feel inspired to do a good deep clean of your lab scales. We can’t help you sing like Snow White so that animals come help you clean but we can help you get your scales […]

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In Basic Lab Skills & Know-how 22nd of June, 2015
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Get To Know Your DNA Polymerases

While most may think standard Taq is the backbone of PCR, there are many other DNA polymerase options out there. The polymerase you use has a significant impact on the efficacy of your PCR, specifically on the product yield, the purity of the product and the faithfulness with which the starting product is transcribed. Sometimes, […]

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In PCR, qPCR and qRT-PCR 18th of June, 2015
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Fuzzy Wuzzy Problems: Achieving and Maintaining a Contamination-free Incubator

When you share an incubator with a number of people it can be very hard to keep a clean shop and months, or more, of work can be lost due to contamination. The two biggest sources of bugs in an incubator are: the water pan the containers being put in and out. Both of these […]

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In Cells and Model Organisms 17th of June, 2015
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Problems amplifying GC-rich regions? Problem Solved!

No, it isn’t you that’s the problem, and you’re certainly not alone if you’re having trouble amplifying GC-rich sequence and/or understanding why GC-rich sequences are causing such problems in the first place! Amplification of GC-rich sequences by PCR has been an irritant for scientists for decades! When we say “GC-rich” we mean ?60% of the […]

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In Lab Statistics & Math 26th of May, 2015
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Capillary Gel Electrophoresis: An Alternative to SDS-PAGE?

When you think about separating proteins, do you think about separating them using a gel? Specifically using SDS-PAGE? If you answered “yes”, it is for good reason. SDS-PAGE is ubiquitous in molecular biology labs because it is good at separating proteins. However, SDS-PAGE takes a lot of time and is labor-intensive. So let’s expand your […]

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In Protein Expression & Analysis 19th of May, 2015