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Troubleshooting Surface Plasmon Resonance: Resolving Non-Specific Binding, Regeneration and Other Problems

Surface plasmon resonance (SPR), a label-free, real-time way to examine protein binding and other molecular interactions, is getting easier as manufacturers have streamlined SPR instruments and supporting software. But problems can still arise. Troubleshooting Your SPR Assay Here are some common issues and suggestions to solve them: Inactive Targets Your target protein may have become […]

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In Lab Statistics & Math 30th of November, 2016
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Ten Ways to Give Your Surface Plasmon Resonance Experiments a Hand

Surface Plasmon Resonance (SPR) is the gold standard for measuring biomolecular binding without the need for labeling (i.e., label free detection of kinetics). SPR is especially valuable because it doesn’t just provide information at the start and end of a binding event, but can be used to follow association and dissociation kinetics of biomolecules in real-time. […]

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Western Blot, ELISA, SPR, or PCR: Which Technique Should I Use?

A pretty standard experiment in a molecular biology laboratory is where a scientist stimulates some cells/tissue with a stimulus of interest (e.g., a cytokine). They can then analyze the cellular response to the external stimulus and investigate what intracellular signaling pathways have been activated/deactivated or what proteins have been secreted from the cell as a result. […]

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