rtirabassi

Rebecca S. Tirabassi received her Ph.D. in Molecular Biology studying the pathogenesis of herpesviruses. After her postdoctoral fellowship, she spent 6 years working in biotech followed by 3 years as a scientist at academic institutions. She is currently the owner of Scientific Pens, LLC, a company that provides research, writing, editing and consulting services for life scientists. In addition to writing for BitesizeBio, she is the voice behind the WiKi and Blog at Advansta.com. She also writes a personal blog entitled Mad Scientist.Crazy Mom in which she talks about surviving life with 5 kids and a menagerie of animals while trying to keep her scientific mind happy (aka occasionally writing about scientific topics that interest her - like herpesviruses).

Articles by rtirabassi:

The Magic Continues: TOPO Cloning

They say a magician is never supposed to tell the secret behind his tricks.  It’s a good thing then that I am a molecular biologist and not an illusionist.  Because once I learn a good trick, I feel the need to spread it like dandelion seeds floating on a breeze. That’s what inspired me to…

20 Jun 2014 DNA / RNA Manipulation and Analysis

How to quality control check your RNA samples

You finally have your RNA in hand.  Now what? The success of downstream applications, such as microarrays and quantitative RT-PCR, relies upon having high quality, intact RNA. So it is worth your while to make sure you have a good RNA preparation before jumping into your experiments. There are three things you want to check…

21 Mar 2014 DNA / RNA Manipulation and Analysis

Ta-Da! The Magic of Taq and TA Cloning

Sometimes a clever little trick for cloning comes along that makes you just give an appreciatory “ahhh.” For me, it was TA cloning.  TA cloning is not a new technique (I am showing my advanced laboratory age here), but when I discovered it, the simple elegance of the technique made me pause and wonder about…

07 Mar 2014 DNA / RNA Manipulation and Analysis

Buying a Secondary Antibody: Why all the Choices?

So you grab a quick 5 minutes in between lectures to sit down and tackle an item on your to-do list: order a secondary antibody for an upcoming experiment.  But when you start to search your favorite secondary antibody provider’s website, you realize it is not going to be a 5 minute job.  Conjugated, F(ab’)2…

03 Mar 2014 Basic Lab Skills & Know-how

Brahe’s Battles: The Outcome

What do science and rapping have in common?  Usually not much.  Unless you happen to be Tom McFadden that is, and then rapping becomes a tool for teaching kids about science. Tom McFadden is no stranger to music and science.  Tom is a science communicator whose hit single, Regulatin’ Genes, garnered him national recognition.  In…

24 Feb 2014 Science Communication & Ethics

Counting On Your Results – Tips And Technologies For Colony/Plaque Counting

Have you ever emerged from the lab, bleary-eyed, blinking dazedly at the sun after spending hours hunched over a lab bench counting endless bacterial colonies or viral plaques? A necessary evil… I consider colony/plaque counting one of the necessary evils of working with microorganisms.  Necessary because many experiments have an endpoint that requires determining the…

03 Sep 2013 Protein Expression & Analysis

What is Sterile? Find Your Way around a Sterile Tissue Culture Hood

You’ve been told that maintaining a sterile environment in a tissue culture hood is vital to preventing contamination of cell cultures. But what exactly is meant by sterile? The definition of sterile is ‘completely clean, sanitized, and free of all forms of life’. Obviously you still want your cells and/or any other organisms you are…

07 Aug 2013 Cells and Model Organisms

RNases can be Useful Too

Ribonucleases (RNases). We’ve been giving them a hard time here lately. We’ve been talking about how they are the bane of the lives of  researchers who work with RNA. And we’ve told you how to find their weaknesses and shown you a myriad of weapons that you can use to kick their RNase butts. But…

09 Apr 2013 DNA / RNA Manipulation and Analysis

Surviving the Big Chill: Freezing and Thawing Mammalian Cells

Cryopreservation is crucial to long-term maintenance of cells. Not even the most diligent and dedicated scientist is capable of maintaining a cell line day in and day out for years. Additionally, cells can change properties, senesce, or, ooops (!) become contaminated as the length of time in culture increases. Freezing and resuscitating your cells can…

03 Apr 2013 Cells and Model Organisms

Cell Culture on a Budget: Homemade Fixes

Mammalian cell culture can easily deplete grant funds and a lot of companies are eager to help you with this! But you don’t always have to buy fancy equipment or hot new reagents to get the job done. Sometimes you can alter protocols to use expensive reagents sparingly. Here are a few tips I have…

06 Mar 2013 Cells and Model Organisms