Rachael Walker's Profile

MIFlowCyt Guidelines read on

MIFlowCyt Guidelines: Helping You to Publish Your Flow Data

Wow, you’ve done it! Your experiment worked and your boss asked you to write it up for publication in your favorite journal. Where to start with presenting your flow data? Take a deep breath, help is in hand in the form of the MIFloCyt Guidelines. As with other scientific techniques, there are ‘Minimum Information about […]

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In Flow Cytometry 9th of July, 2016
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Alternative Careers: Day in the Life of a Flow Cytometry Core Facility Manager

I have been working and managing flow cytometry core facilities in Cambridge for 10 years and I would like to share with you some of my experiences. I have worked up the career ladder in the past 10 years and in 2012 I became the Head of Flow Cytometry at Babraham Institute. This means that […]

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Ain’t no mountain high enough: Summit Software

The town of Fort Collins is situated in the foothills of the Rocky Mountains in Colorado and it is those mountains that give inspiration to the name of the Beckman Coulter acquisition program Summit. Summit software started out as the software to run the MoFlo (Modular Flow) high-speed cell sorter that was designed by Ger […]

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In Flow Cytometry 9th of July, 2016
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Spot the Difference: 5 Ways to Improve the Presentation of Your Flow Cytometry Data

Take a look at the dotplot below, are you happy with the way it’s presented? Do you think that you could recreate that experiment? If you were a reviewer, would you accept that figure? Sure, it’s flow plot, it shows 3 populations of which two are gated. Read many journals and you will see data […]

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In Flow Cytometry 9th of July, 2016
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Troubleshooting: No Events on Your Cytometer

It’s happened to us all, you are ready to run your samples on the cytometer and you can’t see your cells on the screen. Here are a few tricks to troubleshooting this: Cytometer vs. computer connection The different types of cytometers will need different orders for switching on the cytometer and computer. Some are cytometer […]

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In Flow Cytometry 9th of July, 2016
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5 Ways to Improve Your Fluorescent Protein Sorting

If, like many people, you use fluorescent proteins to view your transfection efficiency or your CRISPR gene editing, you can also isolate cells with different expression levels of fluorescent proteins. Here are 5 ways to improve your sorting experiment.

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In Flow Cytometry 9th of July, 2016
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A Guide to Flow Cytometry Software: Becton Dickinson’s ‘Diva’

If you use a Becton Dickinson (BD) cytometer in your lab, the chances are you are acquiring your data using ‘Diva’ software. Diva software is used to acquire your cytometry data on LSRII, LSRFortessa, CantoII and Aria cell sorters. As well as acquiring your data using Diva software, you can also analyse your data after […]

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In Flow Cytometry 19th of May, 2015
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10 Hints For Setting Up A Core Flow Cytometry Lab

If you are establishing a flow core facility or even setting up just one cytometer, then you need to know a few things before you plan your new lab and start looking at purchasing cytometers: Before You Start 1. Know Your Customer It’s really important to know if you are going to be setting up and […]

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In Flow Cytometry 7th of April, 2015
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Shapiro’s Laws of Flow Cytometry

Once you have been in the field of Flow Cytometry for a bit, the name Howard Shapiro will be familiar to you. However, for those who are new to Cytometry, you might not be aware of Dr Shapiro and his fabulous book ‘Practical Flow Cytometry’. Most Cytometrists have a paper copy of this bible of Flow […]

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In Flow Cytometry 3rd of March, 2015
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All sorted? Getting the best out of your cell sorting facility

If you are bringing cells to a core to be sorted there are a few things you can do to optimise your sorting and will help the core staff. Here are a few hints: 1) Know the size of your cells Do you know the size of your cells?  Not many people do and its one of […]

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In Flow Cytometry 12th of December, 2014