Nikos Parisis's Profile

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How Good Is Your Best Friend? A.K.A. Have You Validated Your Antibody?

You use your antibody frequently, maybe even every day. You use your antibody in your Western blots, immunohistochemistry, FACS, ELISA, and pull downs. You’d be lost without it. But how well do you really know your antibody? Are you sure that it detects what you think it detects? If your answer is “dunno” or “not […]

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In Protein Expression & Analysis 5th of February, 2015
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Get Out of Western Blot Hell: An Intro to Mass Spectrometry

After you finish immunoprecipitating a protein or purifying a subcellular compartment, you need to identify what proteins you purified. You could attempt to identify your purified proteins the old fashioned (and slow!) way by running a multitude of Western blot. But rarely do labs have unlimited funds for Western blot antibodies. And lets face it, […]

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How to Take a Cell Apart, i.e. How to Perform Subcellular Fractionation

  I do not have to tell you that eukaryotic cells are not just sacks of homogeneous protoplasm. Instead the inside of all eukaryotic cells are divided into organelles and compartments, each with unique functions and unique protein populations. And there may come a time when you need to separate these organelles and their protein […]

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