Niki Athanasiadou's Profile

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From ChIP-seq to MeDIP: A Glossary of Different NGS techniques

It was not long since the commercialization of NGS (a little more than ten years ago) that scientists went beyond the basics and got creative with the new technology to study much more than just the sequence of DNA. In this article we highlight some of the different NGS technologies and methods available out there. […]

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In Genomics & Epigenetics 20th of March, 2015
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A Quick-Fire Guide to Shotgun Sequencing (and Assembly)

“Making the simple complicated is commonplace; making the complicated simple, awesomely simple, that’s creativity.” –  Charles Mingus   Next Generation Sequencing (NGS) technology has boomed in recent years, allowing researchers to probe further into the workings of the genome. According to the theory of simplicity, it is the simple principles on its basis that make […]

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In Genomics & Epigenetics 6th of March, 2015
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Sequencing the microbiome: re-discovering our invisible allies

Scientific breakthroughs have profound effects on how we view and treat the world that surrounds us. Especially in the microscopic level, scientific discoveries provide the only understanding we have of Nature. One such case is the story of our relationship with microorganisms. First impressions Since Luis Pasteur’s discoveries on invisible “infectious agents” more than 150 […]

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In Genomics & Epigenetics 11th of September, 2014
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NGS and TRex: The Science Of Jurassic Park

The necessity to see negative scientific results in academic journals has recently drawn some attention. However, for the working experimentalist, it is still unthinkable that he or she would be able to publish meticulous experiments that have painstakingly produced, well, nothing! That is unless Hollywood has made your experiment part of the popular culture. A satisfying […]

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In Genomics & Epigenetics 26th of September, 2013
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Solid Phase Reversible Immobilization: How To Get A Bead On The Clean-up Of Your NGS Libraries

“Any sufficiently advanced technology is indistinguishable from magic.” – Arthur C. Clarke In the fast-moving field of next generation sequencing, standard practices are evolving rapidly. Today, more and more labs are using Solid Phase Reversible Immobilization (SPRI) beads instead of gel purification in the preparation of libraries for sequencing. A crucial step, not for the […]

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In Genomics & Epigenetics 13th of June, 2013
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Celebrating The 60th Anniversary Of The Publication Of DNA Structure: Epigenetics And NGS Pave The Way Forward

Today, the 25th of April 2013, marks the 60th anniversary since the groundbreaking publication on DNA structure by James Watson and Francis Crick. In recent years we are witnessing another revolution in biology; epigenetics. And, like figuring out the structure of DNA required the development of X-ray technology, so epigenetics is intimately linked with next generation sequencing. […]

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In Genomics & Epigenetics 25th of April, 2013
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Ignore The Ticking Bomb At Your Peril: RNA-Seq Normalization- A Lurking Problem And It’s Solution.

You have spent days, if not weeks, at the bench setting up the treatment and control samples for that crucial experiment. You submitted your cDNA library for sequencing and after a few weeks of waiting anxiously you get back a list of differentially expressed genes. Hooray?! Hold on- not quite yet! There is something you […]

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In Genomics & Epigenetics 14th of March, 2013