Dr Nick Oswald's Profile

Sponsored Education
read on

Baby’s first DNA model

I wouldn’t normally expect to include an article on knitting in a molecular biology blog, but Kimberly Chapman’s DNA model for babies is so cool (in a very geeky way) that I thought it deserved a mention. I’d like one of these, but unfortunately, they are not commercially available. Kimberley only provides patterns on her […]

read on
In Fun Stuff 1st of September, 2007
Sponsored Education
read on

Better Pubmed Journal Searches

Ever get too many hits from your Pubmed searches? Using field tags allows you to generate more specific searches than keywords alone, saving you from trawling through hundreds of irrelevant articles.

read on
In Taming the Literature 31st of August, 2007
Sponsored Education
read on

DNA replication, transcription and translation in action

While browsing you tube I came across this really nice animation of DNA replication, transcription and translation complete with imaginative sound effects. I hope you enjoy it as much as I did.

read on
Sponsored Education
read on

Low cost DNA gel documentation

Equipment for photographing DNA gels stained with ethidium bromide (or other fluorescent dyes), doesn’t have to cost thousands of dollars. These days, great pictures can be obtained with a standard digital camera and an orange filter. Here’s how.

read on
Sponsored Education
read on

My favorite PCR polymerase

What do you get when you cross a Pyrococcus DNA polymerase with a dsDNA binding domain? …a highly processive, high fidelity, lighting fast PCR work-horseThose Finnish wizards at Finnzymes have made that work-horse a reality with their Phusion polymerase. I had been meaning to try out this enzyme for quite a while, but never got […]

read on
In PCR, qPCR and qRT-PCR 28th of August, 2007
Sponsored Education
read on

Plasmid Cut & Paste with Quikchange

I recently had a problem where I needed to cut out an expression cassette (a promoter coupled to a coding sequence) from one plasmid vector and paste it into another containing an expression cassette so I could get tandem expression of the two genes. The recipient vector had no suitable restriction sites for performing this […]

read on
Sponsored Education
read on

Zyppy Plasmid Miniprep Kit

Zymo research have launched a new plasmid miniprep kit that not only is has the coolest name of any kit on the market but is, the manufacturers claim, the fastest means of plasmid purification available.

read on
Sponsored Education
read on

RNA Polymerase II Assayed in Living Cells

A Nature Structural & Molecular Biology article published by Singer et al has provided a fascinating insight into the kinetics of RNA polymerase II during transcription.

read on
In Of Interest 24th of August, 2007