Dr Nick Oswald's Profile

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Get Your Clone 90% Of The Time with Ligation Independent Cloning

Are you stuck in cloning hell?, Tired of doing ligations that don’t work? Want a faster, more efficient cloning procedure? You should try ligation independent cloning. A growing number of researchers swear by ligation independent cloning methods because they are simpler and more efficient than conventional cloning and as a recent convert to their ranks, […]

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What’s The Problem With Ampicillin Selection?

Ever wonder what those small colonies, like satellites, surrounding a larger E. coli colony on your LB with ampicillin plates were? Or why, when you picked that colony, it never had the plasmid you just transformed? Well, it’s because those satellite colonies are “protected” from the ampicillin by the big colony. Read on for more… Ampicillin […]

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DNA ligation read on

5 DNA Ligation Tips

DNA ligations can be frustrating. Sometimes they just don’t work, for no obvious reason. My top 5 DNA ligation tips will help to improve the efficiency of your ligation reactions and hopefully give a better cloning success rate. 1. Aliquot the Ligase Buffer The ATP in the ligase buffer is essential for the DNA ligation […]

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PCR Problems? Try an Additive

You’ve tried all the usual stuff, and checked the primer sequences twice, but still can’t get that PCR fragment amplified. It’s time to enter the strange world of PCR additives. Over the years a variety of additives have been shown to enhance PCR reactions in certain situations. Here is a summary of some of the […]

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In PCR, qPCR and qRT-PCR 9th of July, 2016
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optimal temperatures read on

Why Do Enzymes Have Optimal Temperatures?

Every biologist is familiar with the profile of the rate of an enzymatic reaction versus temperature as shown in the figure. We know that enzymes from E. coli or warm-blooded animals tend to have an optimum around 37°C, while those from thermal vent bacteria have much higher optimal temperatures. Surprisingly, I find that many biologists […]

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How to Shut Off Background Lac Expression in LB

Here’s a tip that you may find useful if you are expressing proteins in E.coli using a lac promoter-based expression system, e.g. pET, in LB medium (L-broth). Lac expression systems are typically induced in the lab using IPTG (isopropyl-beta-D-thiogalacto- pyranoside), which is a non- hydrolyzable analog of lactose, the natural inducer of the lac operon. […]

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How To Be Happier and More Productive in Your Work

In this webinar, presented by Bitesize Bio founder, Nick Oswald, you will learn: How going with the crowd is toxic to your happiness in science (or any career), and what to do about it. The role your habits play in shaping your productivity and happiness, and how to form habits that serve you. How a […]

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In Personal Development 10th of November, 2015 Presented by: Dr Nick Oswald
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5 Ways to Clean Up A DNA Sample

One of the most common tasks in molecular biology is cleaning up DNA from aqueous solutions to remove buffer salts, enzymes or other substances that could affect downstream applications. Examples include cleaning up PCR reactions, digests or other enzymatic treatments and cleaning up genomic or plasmid DNA contaminated with cellular proteins/debris. There are several ways […]

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Fast-track your Ampicillin Plasmid Transformations

Most of us use pretty standard transformation protocols for E.coli. Yours probably goes something like this: – Thaw the competent cells on ice – Add DNA – Electroporate (or incubate then heat shock for chemically competent cells) – Add rich medium (LB or SOC) – Incubate at 37°C (or appropriate temperature) for 30-60 minutes – […]

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Webinar
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Five Principles for Creating the Career of Your Dreams

In this webinar you will learn: How to decide whether the academic career path is for you The key mindset required to keep moving your career in the direction of your desires How Nick Oswald evolved his career from researcher to owning and running the business of his choice, and what you can learn from […]

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In Career Development & Networking 18th of May, 2015 Presented by: Dr Nick Oswald