Marisa Fernández-Cachón's Profile

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Ten Tips for Turning Beastly Western Blots Beautiful

Western blots can be ugly. I mean down-right, horrifically, wall-of-shame ugly.  Not only can they be embarrassing to show to your colleagues, but the ugliness can obscure your results, making it impossible to interpret your data. Blotting consists of many experimental steps, which makes the technique naturally error-prone. Although standardized protocols exist, many fail to […]

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Banish Those Insecurities: Tips on Giving Scientific Talks

Every once in a while you are forced to abandon your comfort zone.  You have to leave behind your comfortable territory of the lab bench or your desk in the reading room and stand up in front of a group of people. Then, you will be expected to deliver – the department seminar, a progress […]

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An Easy Way to Start Using R in Your Research – Introduction

Working with large datasets can be very frustrating and time consuming.  If only there were more tools out there to simplify things without needing to invest a PhD’s worth of time to learn how to use them! I am here to tell you that there is a solution, and a free one at that. If […]

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A Step-by-Step Guide to Designing qPCR Primers

Primer design is a very important step when setting up your qPCR or reverse transcription-qPCR assay (RT-qPCR). If your qPCR primers anneal poorly or to more than one sequence during amplification, it can significantly impact the quality and reliability of your results. Also, if you are performing a one-step RT-qPCR, the reverse transcriptase will use […]

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In PCR, qPCR and qRT-PCR 6th of February, 2013
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The Pros and Cons of a PhD or Post-doc in a Foreign Country

It’s time to start thinking about your next move. Maybe you’ve finished your undergrad/master’s degree and are considering doing a PhD. Or maybe you’re past all that and need to find a post-doc position. Amongst all of this, you might be considering the option of doing your PhD or post-doc in a foreign country. It’s […]

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In PhD Survival 4th of August, 2011