Lynnea Waters's Profile

optics system read on

Demystifying the Flow Cytometry Optics System: A Peek Under the Hood

To many users, the flow cytometer is a magic box: put in cells, get out data. You click the button to tell it which colors to look at without much thought about how the machine does this. However, not all fluorophores are created equal—some configurations might exclude the spectrum you’re really looking for. Here’s a […]

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In Flow Cytometry 24th of November, 2016
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How to Give a Great Scientific Talk and Engage Your Audience

We have all been to awful talks—hours of slides crammed with data, given by presenters who assume you know as much as they do. But hopefully you’ve also seen a great scientific talk. A talk in which you’re walked through a story, eagerly anticipating each question and data point, until you finally reach a conclusion […]

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In Writing, Publishing & Presenting 14th of September, 2016
cell cycle analysis read on

Cell Cycle Analysis by Flow: DNA Stains and Beyond

While you can observe mitotic cell cycle progression using immunofluorescence, flow cytometry is a great tool to delineate details that aren’t apparent by chromosomal morphology alone. DNA stains are a great way to get a general idea of what your cells are up to. There are also a number of other stains you can use […]

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In Flow Cytometry 8th of September, 2016
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Common Mass Spectrometry Contaminants: I Messed It Up So You Don’t Have To!

Through many trials, and lots of error, I learned that there are many considerations for mass spectrometry that might not be obvious to you as a molecular biologist. Common contaminants, even in small quantities, can mask important peaks in your mass spec data and have a huge impact on the final results.

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In Protein Expression & Analysis 1st of September, 2016
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The Care and Keeping of Primary Murine B Cells

So you want to work with mouse B cells? Primary murine B cells are a difficult, yet fascinating system to work with and can help deepen your understanding of an immunological system. You can study many things with primary B cells, including: immune activation antibody production cell-cell interactions between immune cells and immune phenotype These […]

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In Cells and Model Organisms 11th of August, 2016