Kristen Haberthur's Profile

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How Fluorescent Molecules Work: Shine Bright like a Diamond

Fluorescence is one of the most important and useful tools in a biologist’s toolbox. In biology, nearly every field, from physiology to immunology, uses fluorescent molecules (aka fluorophores) to detect proteins. However, the specific science behind how fluorescence works can be confusing or overlooked. Have no fear! In this article, we break down key points of […]

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In Flow Cytometry 27th of January, 2017
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Transitioning out of the Lab: B­­­­reaking up Is Hard to Do

Working in a research lab is not a normal job. The hours are often unconventional and the tasks can run from exciting to mundane—it’s a world all of its own. Even so, your loyalty to your field and people is unmatched; there is a level of comradery you experience that is unlike any other. This […]

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In Survive & Thrive 12th of December, 2016
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The Art of Scientific Authorship: Political Science

The elusive manuscript. It’s what we, as scientists, build our kingdoms on—throwing ourselves into our research, hoping to feel our time in the sun when it all comes to fruition in the form of that glorious body of work. But…what how do you determine who should share in that sunshine? Should you always put your […]

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The History and Future of Fluorescent Labels: We’ve Come a Long Way, Baby!

If you’ve been keeping up with our recent series of articles, welcome back! If not, you can catch up on how fluorescence works or what not to do with your flow experiment. In short, we have been discussing fluorescent labels and their role in flow cytometry. Today, I’ll round out our discussion by touching on […]

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In Flow Cytometry 8th of November, 2016
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Corralling Your Cells: How to Gate in Flow Cytometry

Flow cytometry. Some people love it—most hate it—but all can agree that it is one of the most powerful analytical tools immunologists possess. Here’s a quick refresher: as the name suggests, flow cytometry measures the physical and chemical characteristics of cells. This is accomplished by fluorescently labeling cell surface markers/proteins using antibodies conjugated to fluorophores. […]

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In Flow Cytometry 27th of October, 2016
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Lighting the Way: Understanding Flow Cytometry Fluorophores

As science is becoming more interdisciplinary, the tools we use to answer questions are also crossing party lines. Case in point: flow cytometry. Once a tool only used by “real” immunologists, flow cytometry is fast becoming a method by which numerous questions can be answered, from the length of a cell’s telomeres, to the state […]

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In Flow Cytometry 18th of October, 2016
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Science Outreach: Why Should You Care?

All scientists should be involved in some aspect of outreach. There. I said it. I know, I know. This goes completely against why most scientists pursued their careers in the first place: to dedicate their lives to discovery, and to do so alone. With minimal human interaction, especially with non-scientists. Why You Should Reach Out […]

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In Science Communication & Ethics 28th of September, 2016
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How to Destroy your Flow Cytometry Data in 3 Easy Steps: Snap, Crackle, and Pop

While many scientists are methodical and precise, some of us like to live on the edge. Read a protocol all the way through? No thanks, I’ll take my chances and guess what concentration of HCl I should use. Label my tubes with the correct content? Puh-lease – it’s much more exciting deducing which is which […]

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In Flow Cytometry 18th of August, 2016
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Catching Greatness: Measuring Cellular Degranulation

One of the key characteristics of cytotoxic cells (i.e. CD8+ T cells, natural killer cells) is the presence of pre-formed cytoplasmic lysosomal granules. These structures house perforin and granzyme; two molecules that are essential for the lysis of target cells. Upon effector cell activation, granules are polarized toward the target cell and the contents are […]

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In Flow Cytometry 9th of July, 2016
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Pregnancy and Post-docing: A Case Study

Deciding to start a family with your partner is one of the most exhilarating and frightening times you’ll likely ever experience, outside of welcoming the little F1 to the world that is. Your job should not be an inhibitory factor in making that decision. We all know that post-docs live in a purgatory of sorts: […]

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In Personal Development 9th of July, 2016