Kimberly Jasmer-McDonald's Profile

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Faster PCR Optimization

So, you’ve designed PCR primers to amplify your sequence of interest, and you’re ready to go.  But unless you have a never-ending supply of template, polymerase, and a thermocycler with a gradient function—not to mention a hefty dose of time and patience—you probably don’t want to spend the next week finding the perfect conditions for […]

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In PCR, qPCR and qRT-PCR 15th of December, 2016
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How to Fix Your Bad Cloning Ratios

You open the incubator in the morning and to your dismay there are a hundred glorious colonies… on your vector-only control plate. While there are a number of potential causes, I’ll highlight a few of the more likely culprits and their solutions.

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How to Communicate Your Way to Scientific Success

As a new postdoc, I found myself in an uncomfortable situation. In my new environment, I am constantly in contact with my mentor and fellow lab members. I must regularly communicate with them and I have routine access to a sounding board for advice and troubleshooting. While I recognize the value of this situation, I initially found it difficult to navigate.

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