So, you’ve designed PCR primers to amplify your sequence of interest, and you’re ready to go. But unless you have a never-ending supply of template, polymerase, and a thermocycler with a gradient function—not to mention a hefty dose of time and patience—you probably don’t want to spend the next week finding the perfect conditions for […]
As a new postdoc, I found myself in an uncomfortable situation. In my new environment, I am constantly in contact with my mentor and fellow lab members. I must regularly communicate with them and I have routine access to a sounding board for advice and troubleshooting. While I recognize the value of this situation, I initially found it difficult to navigate.