Dr. Karen O'Hanlon Cohrt's Profile

Webinar
view video

Multiplex PCR Technology: What Is It All About?

Join Dr. Karen O’Hanlon Cohrt for a practical tour of multiplex PCR technology, where you will learn the following and much more: Principle behind multiplex PCR technology Popular applications of this technology How to set up this reaction Advantages and disadvantages Multiplex PCR Can Benefit Your Research Dr O’Hanlon Cohrt will discuss the history of […]

read on
In PCR, qPCR and qRT-PCR 1st of December, 2016 Presented by: Dr. Karen O'Hanlon Cohrt
read on

Introducing You to the Wonderful World of Microbes!

Welcome to the microbe series where we have a very exciting line-up planned over the coming months. Here we will talk about everything microbial, including the uses of microbes in industry and medicine, emerging pathogens, diagnostics, and much, much more! Let’s kick off this series with an introduction into these wonderful, yet sometimes nasty, organisms […]

read on
read on

Mysterious miRNA: Identifying miRNAs and Their Targets

In my first article on this topic we delved into what miRNAs are, how they are generated, and their function. Now, we are going to discuss how to identify miRNAs and their targets. Why do you want to look at something so small anyhow? miRNAs play a crucial role in most physiological processes. It’s not […]

read on
Arabidopsis thaliana: the species in which plant miRNAs were discovered read on

Mysterious Plant miRNAs: What About Them?

Welcome to the last article in this series! Last, but by no means least, we will look at the importance of plant miRNAs and how they differ from their animal counterparts. When/How Were Plant miRNAs Discovered? Plant miRNAs were first described in 2002, a decade after the seminal miRNA study in the nematode C. elegans […]

read on
Webinar
qPCR view video

qPCR Tips: Workflow, Applications and Troubleshooting

In this webinar, you’ll get: Practical advice for sample preparation, qPCR setup and result analyses Guidance on choosing the correct fluorescent labeling system for your qPCR Tips to troubleshoot the most commonly encountered issues in qPCR Join Dr. Karen O’Hanlon Cohrt for a practical tour of the process, applications and practice of qPCR, from start […]

read on
In PCR, qPCR and qRT-PCR 9th of June, 2016 Presented by: Dr. Karen O'Hanlon Cohrt
read on

Understanding Plasmid Incompatibility

Welcome to the last article in the series on E. coli origins of replication where we will touch upon plasmid incompatibility! This is a broad and complicated topic so we will mainly focus on points that might be relevant for you in the lab. What is Plasmid Incompatibility? Plasmid incompatibility is usually defined as the […]

read on
read on

Mysterious miRNAs: An Introduction to MicroRNAs

mRNA, miRNA, siRNA, tRNA, rRNA! Just what do all these RNAs do? Most biology graduates will have heard a good deal about mRNAs, tRNAs and rRNAs since these are vital players in protein synthesis. For siRNAs there has been a lot of focus within drug discovery and biomedical research over the last decade, but there […]

read on
In Of Interest 4th of March, 2015
read on

How to Manipulate Plasmid Copy Number

So far in this series, we have looked at origins of replication, we’ve discussed how plasmid replication is regulated in the popular pBR22 plasmid, and we’ve seen how a disturbance of this regulatory mechanism has given rise to the high-copy pUC18 plasmid. Are you ready for more plasmid talk?? If so, keep reading, as we […]

read on
In Of Interest 14th of January, 2015
read on

qPCR: RNA quality and why it matters

Gene expression analysis plays a pivotal role in a wide range of studies, including biomedical analysis and diagnostics. Of all the methods available for gene expression analysis, quantitative real-time PCR (qRT-PCR) is the most rapid, sensitive, and accurate to measure mRNA, and its use in clinical diagnostics is rising steadily. RNA quality entails both purity and […]

read on
In PCR, qPCR and qRT-PCR 18th of December, 2014
read on

pUC18 – Probably the Best High-Copy plasmid in the World!

Today I would like to introduce you to pUC18, a plasmid most noted for its high copy number. In the first article in this series, we talked about how origins of replication (ori) control plasmid replication and copy number. To learn about this, we focused on the pBR22 ori and the role of Rop protein in […]

read on
In Of Interest 26th of November, 2014