John Mackay's Profile

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Let’s Get Melting

Amplify them all and let the melting sort ‘em out. OK perhaps my attitude to analysing sequence variation may be a little flippant but it characterises a lot of my thoughts toward High Resolution Melting Analysis (HRMA). It is now more than a decade since melting analysis to characterise PCR products was introduced. This technique, […]

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In PCR, qPCR and qRT-PCR 16th of June, 2010
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How To Get a Perfect Pellet After DNA/RNA Precipitation

If you’re performing DNA/RNA precipitations, you will have read Suzanne’s excellent article on which alcohol to use for precipitating your precious samples (check out some useful info in the comments for that article as well). Its publication prompted the recall of a useful tip I learned from a post-doc many years ago,  one of those […]

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In DNA / RNA Manipulation and Analysis 25th of January, 2010
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PCR vs qPCR Sensitivity

One of the great features of PCR is its excellent sensitivity as we know. And many articles describe real-time qPCR as an added leap forward in that sensitivity – to the point where it has become a standard feature of a new assay description Indeed, I’m currently developing a new qPCR assay to replace a […]

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In PCR, qPCR and qRT-PCR 14th of October, 2009
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PCR Rescue: Making One Band From Many

It’s the molecular biologist’s version of ‘I have good news. . and bad news’. The good news is that I amplified the DNA band of interest. The bad news is that I amplified these other bands as well! Oh, and this smear. What to do? Typically you might try and cut out the band of […]

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In DNA / RNA Manipulation and Analysis 14th of September, 2009