jjankovic

Articles by jjankovic:

Divide and Conquer: How to Setup Your First Droplet Digital PCR Experiment

Droplet digital PCR?  It’s easy. Because we’re here to guide you through it. We recently introduced you to the principles of digital PCR technology and how it differs from qPCR. In a nutshell, digital PCR is an end-point PCR technology that divides a single PCR into a large number of partitions, and then perform PCR…

06 Apr 2017 PCR, qPCR and qRT-PCR

Reverse Transcription: The Most Common Pitfalls!

Good quality starting material is king for reverse transcription! Obtaining reliable results in any experiment requires good preparation. We often take reverse transcription for granted, and we don’t always consider that our qPCR might be performing poorly because of problems in that step. Since it’s quite often the reverse transcription reaction itself that causes fuss…

19 Jan 2017 PCR, qPCR and qRT-PCR

Acid Phenol Chloroform Extraction of DNA, RNA and protein: 3 in 1

In austerity times, nothing is in excess. Apart from saving reagents, which can be refilled with extra financial injections, there is a commodity that cannot be easily resupplied – tissue samples! If, like me, you have experienced the fear of not having enough sample for performing a qPCR, western blot, and conventional PCR from the…

23 Nov 2016 DNA / RNA Manipulation and Analysis

Methods for Relative Quantification of qPCR Data. Yes, There is More Than One.

As all of you probably know, methods for calculating relative gene expression from qPCR data include: a) double delta Ct (ΔΔCt) and b) that one other method. Chances are you’ve probably gotten beyond the ΔΔCt method, but you should be prepared in case you face primer sets of different amplification efficiencies. Both methods require the…

18 Aug 2016 PCR, qPCR and qRT-PCR

Choose the Statistical Package that Will Make Your Data Talk

In the last years, the need for using statistical testing in bioscience has grown exponentially and so has the development of statistical software. It is now common that everyone is using some sort of stats in their basic research. Among the skillful biostatisticians, R is the most popular software for data analysis, but not all…

09 Jul 2016 Lab Statistics & Math

Online Schools for Learning How to Play in the R Coding Arena

So, you come from a non-coding background, but given the rapid growth of your research results, you have accrued a pile of data that needs data mining. Now, you can leave this to your bioinformatics core facility, but if you’re curious to understand how they arrive at their conclusions (or you don’t have a BI…

09 Jul 2016 Software & Online Tools