Dr Jennifer Redig's Profile

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Protocols for Cloning Without Restriction Enzymes or Ligases

If you think all cloning techniques require restriction enzymes or ligases, think again. There are lots of ways you can clone without restriction enzymes or ligases for seamless cloning results. Read below to learn about Topoisomerase cloning, SLIC and Gibson. Method #1: Topoisomerase Technology One of my very favorite restriction enzyme-less cloning methods is the […]

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Slides 101

Would you eat your spaghetti dinner without a plate? No, of course not! It would make a big mess and be ugly to look at. Instead you NEED something to put that spaghetti on, to contain it, to keep it clean, to make it look nice – a bowl, a plate, SOMETHING! By the same […]

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In Microscopy & Imaging 31st of March, 2015
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Digital Pathology – why you need it and how to choose the best camera for it

Yup, we really are in the digital age…even the pathology is digital. Facebook, Instagram, Tumblr. It has never been easier to take pictures and share them. The digital revolution is upon us and nothing is safe, even your pathology samples. Digitizing your pathology samples can help you better organize and manage pathology for later. And […]

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In Microscopy & Imaging 30th of October, 2014
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The Devil is in the Details: How to Setup a PCR Laboratory

There is a right way and a wrong way to set up a PCR laboratory. Because of PCR’s tremendous ability to amplify small quantities of DNA/RNA template, even the smallest of template contamination can become a huge problem in PCR. However, contamination does not have to be a problem in your laboratory. Read below to […]

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In PCR, qPCR and qRT-PCR 1st of August, 2014
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Keeping ’em Alive! Incubators and Chambers for Live-Cell Imaging

Live-cell imaging is a powerful technique that allows you to image dynamic cell processes, such as protein trafficking, signal transduction, endocytosis, and exocytosis, etc. However, to perform your own live-cell imaging you need the right equipment, most notably a live-cell imaging chamber or incubator. Keeping ‘em alive! Live-cell imaging differs from conventional imaging in that […]

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In Microscopy & Imaging 6th of May, 2014
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Just What Do All These Additives Do?

Every PCR battle is the same: Too little amplification of your target DNA versus too much amplification of off-target DNA. But you can win the PCR battle and amaze your co-workers by mastering the use of PCR additives. PCR additives usually work one of two ways: 1) By reducing secondary DNA structures and thus increasing […]

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In PCR, qPCR and qRT-PCR 4th of April, 2014
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“Where the hell did my signal go?” AKA The Problems (and Uses) of ‘Photobleaching’ in Microscopy and Imaging

Like most things in this world, fluorophores are mortal, and eventually your once bright fluorescent image will inevitably fade to black. This fading or ‘photobleaching’ of fluorescent signal can make imaging difficult, especially if you are trying to take quantitative images. Read below to learn what causes photobleaching of your fluorophores and how best to […]

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In Microscopy & Imaging 4th of March, 2014
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Welcome to the Dark Side…Dark Field Microscopy That Is!

We all know that stars are easier to see against the dark background of the night than they are to see against the bright sky of day. Well did you also know that the same may be true of your microscope specimen? Dark field microscopy is a popular microscope technique that makes your unstained transparent […]

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In Microscopy & Imaging 25th of February, 2014
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What is PCR? – The Beginner’s Guide

PCR is THE technique of modern molecular biology labs. If you need to copy, sequence or quantify DNA , you need to know PCR. In short, PCR (polymerase chain reaction) is a biochemical technique that uses thermocycling and enzymes to quickly and reliably copy DNA, and it was invented in a flash of inspiration by a […]

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In PCR, qPCR and qRT-PCR 27th of January, 2014
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Advanced Sectioning Techniques: How to Section Difficult Tissues.

Has this ever been your experience: You lovingly harvest, fix and embed your tissue, only to have your tissue shatter, wrinkle or otherwise look horrible when you section it? Well, before you throw your microtome across the room (if you can pick it up in the first place!), then read this article. Some tissues are […]

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In Microscopy & Imaging 17th of December, 2013