Jennifer Cable's Profile

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What Can NMR Do For You? — Part Two

Welcome to part two of “What Can NMR Do For You?”, a three-part series in which we see how you can use simple NMR experiments in your research. In part one, we went over some key points to keep in mind when doing NMR on proteins and DNA, such as sample preparation, and saw how […]

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Turn That Frown Upside Down! or, How to Publish Your Negative Results

There are six little words that can instill both excitement and trepidation in the heart of a graduate student: “No one’s ever done this before.” What those words really mean, of course, is “No one’s ever published this before,” and you are either standing at the edge of a great discovery or a chasm of […]

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What Can NMR Do For You? – Part One

Having done my PhD work in an NMR lab, I sometimes feel like NMR doesn’t get the credit it deserves. As a structural tool, it always seems to play second fiddle to crystallography, and not many people seem to know about all of the different types of information that NMR can give you. So why […]

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How to Measure Protein Concentration More Accurately

When working with proteins, one key part of any good assay is accurately determining how much protein you have.  Accurate quantification is obvious important if, for example, you’re trying to determine a binding constant or measure enzyme kinetics; but even if you’re doing something more qualitative, having a good idea of how much protein you […]

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In Basic Lab Skills & Know-how 30th of November, 2011
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How to Design the Perfect Protein Purification Buffer

When purifying a protein, it’s important to keep your protein happy; that usually means that the protein is soluble and active.  Creating a buffer that prevents unfolding and aggregation is therefore crucial to the success of your experiments.  There are several factors that you should consider when designing a buffer: pH, buffering system, salt, reducing […]

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In Protein Expression & Analysis 3rd of November, 2011