Jason Garner's Profile

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Pyromania: An Intro to Pyrosequencing

Anyone who is involved in DNA sequencing in one form or fashion knows there are multiple ways to skin a cat: Sanger-based, next generation (NGS) and of course the new ion torrent sequencing technology. Which technology you use is usually dependent on the questions you’re trying to answer – and how fat your wallet is. […]

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In Genomics & Epigenetics 10th of January, 2013
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!BAM! !POW!: How to Become a Better Job Search Street fighter – Final Round

Up till now, we’ve discussed many different ways we can prepare and execute a new career search. In the last series, I discussed getting away from internet job boards and out into the real world. Now, I’m going to challenge you and push most of you way out of your comfort zone. So, there’s XYZ […]

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In Career Development & Networking 12th of December, 2012
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Kits And Reagents For Mammalian Cell Transfection

Transfection of animal cells has proven an invaluable tool in studies of gene expression, cell behavior, cell processes and molecular genetics. Essentially, transient pores are opened within the cell’s lipid bilayer, allowing insertion of nucleic acids (DNA, RNA, siRNA, RNAi), proteins, nanoparticles, and even antibodies into the cellular milieu. Transfection can be achieved using many […]

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In Protein Expression & Analysis 16th of November, 2012
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The Advanced User’s Guide to Sequencing Alignment Software (Members Only Article)

Whether you’re employing sequencing gels, Sanger-based methods, or the latest in pyrosequencing or ion torrent technologies, obtaining, manipulating and analyzing your sequences has never been easier. Depending on what your goals are, you need to understand the pros and cons of the software. There is a lot of software out there, so do you your […]

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The Beginners Guide to DNA Sequence Alignment

Fortunately, those of us who have learned how to sequence know that aligning sequences is a lot easier and less time consuming than creating them. Whether you’re employing sequencing gels, Sanger-based methods, or the latest in pyrosequencing or ion torrent technologies, obtaining, manipulating and analyzing your sequences has never been easier. We’re going to take […]

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Book Review: “Complications: A Surgeon’s Notes on an Imperfect Science”, By Atul Gawande

At one point in my academic career, I was an eager pre-med student faced with making a difficult decision: MD or PhD? On the one hand, I was interested in orthopedics/sports medicine, and on the other hand, I wanted to be elbow-deep in disease research. One brainiac even suggested pursuing an MD/PhD: a good idea […]

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In Inspiring & Thought Provoking 24th of August, 2012
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!BAM! !POW!: How to Become a Better Job Search Street fighter – Round Two

Thomas Edison, when asked about his failures in creating the world’s first single carbon filament incandescent lightbulb, said (and I’m paraphrasing here): “I didn’t fail, I simply found 2000 ways how NOT to make a lightbulb – and only one way to make it work”. If only most job-seekers possessed this attitude. It’s amazing the […]

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In Career Development & Networking 6th of August, 2012
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!BAM! !POW!: How to Become a Better Job Search Street Fighter – Part One

Career searches and job hunts are painful: answering ads, resumé tweaking, phone calls, interviews, preparation, sweat, tears, fear of rejection, etc. But it’s funny, even after investing all of this effort, the same mistakes are often made by newbies and veterans alike. In this series of articles, we’ll be talking about a variety of ways […]

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Film vs. Digital: A Real Western (Blotting) Showdown

You’ve masterfully run and transferred your gel, and now it’s time to probe and quantify your protein(s). You’ve got your antibodies and ECL ready to go. Substrate – check. Film cartridge – check. Darkroom – ah yes, that magical place where night vision goggles are required to navigate a veritable minefield of potential chaos. It […]

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In Protein Expression & Analysis 27th of April, 2012
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Save the Data! Your Computer May Be Dead, but Your Hard Drive’s Heart Still Beats!

You get that familiar blue screen or an application hangs, usually one that’s been tanking your memory. No keystroke response.  The deer-in-the-headlight look on your face prompts your colleagues to ask, ‘is everything ok?’ while they secretly laugh at you. Rebooting 27 times will not save you now. Your computer has breathed its last. Even […]

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In Organization & Productivity 28th of March, 2012