Declan Dunn's Profile

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Fluorescent Western Blotting: Lowdown and Advantages

In this article, I will introduce you to the world of fluorescent western blotting. Firstly, we’ll find out how it compares to chemiluminescent western blotting. Then, we will learn how infrared fluorescent western blotting can give you truly quantitative and reproducible results. Lastly, we’ll look at the many advantages of fluorescent western blotting, including the possibility to […]

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In Protein Expression & Analysis 11th of April, 2017
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Quantifying & Assessing RNA: TE or not TE?

Red Pill or Blue? Carrying out science often involves many difficult decisions! I see it all the time in RNA protocols – the “gracious” option of using purified water or Tris-EDTA (TE) buffer to dissolve (or elute, if you are using column purification) RNA. When I was trained in assessing RNA using UV spectrophotometry, graduate […]

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Revealing Cellular Dynamics with Millisecond Precision – The New Tool That Turned Electron Micrographs in Motion Picture of Neural Communication

What if you can dissect the cellular dynamics with millisecond precision? What if you can unravel the morphological transformation of a neuron millisecond by millisecond using electron microscopy? Could this be even possible? In this webinar, we will talk about how optogenetics coupled with high-pressure freezing can make this possible. We will discuss how to […]

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In Microscopy & Imaging 29th of March, 2017 Presented by: Frédéric Leroux, Shigeki Watanabe
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Proteomics and Good Mass Spectrometry Data

It is currently possible to analyze thousands of proteins in a single sample using mass spectrometry (MS) and a database of predicted protein sequences, referred to as ‘bottom-up’ proteomics. With this technology, you can measure protein levels and interactions. Also, you can examine changes in post-translational modifications (PTMs) and isoforms (in an unbiased manner). Working with […]

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In Protein Expression & Analysis 14th of March, 2017
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Four Ways to Get CRISPR Reagents Into Your Cells

Do you need to test the effects of mutating a gene in your system?  Then, CRISPR is the way to go. Your first step is to decide on good target sequences. Then, you have to get the two components of CRISPR: the Cas9 nuclease and “guide” RNA (gRNA). Even though you’ve read up on the technique […]

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Benefits of the GUS Gene Reporter System in Plants

Gene reporters enable valuable insight into gene expression. The GUS gene reporter system is one of the popular and common plant reporter systems. GUS,  is short for glucuronidase, an enzyme in the bacterium E. coli. GUS is a good reporter for plants, as it does not occur naturally, and thus, has a low background. With […]

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Automated Cell Counting with a Fluorescent Twist

Cell counting is the bane of existence of many researchers. Countless hours spent in front of the microscope with a haemocytometer on the stand and a manual tally (or “clicker”) in hand can be really daunting. Not to mention that no one will ever double check your count if you don’t take a picture. Those […]

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Phylogenetic Tree Construction Made Easy with Blast & Mega

Your DNA sequence can be put to good use fairly easily with Blast and Mega software. These programs can help in phylogenetic tree construction. You can ask questions like what is the evolutionary relationship between a set of sequences from different species? Or how have certain microbial strains arisen? Blast As any bioscientist probably knows, […]

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In Genomics & Epigenetics 7th of March, 2017
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Breezing Through the Aftermath of a Conflict

When working very closely with a team of people in the lab, you will have disagreements leading to conflict. That’s just human nature, hence the origin of a common phrase, “Can’t we all just get along?” If you are lucky, you will be able to resolve the conflict. But then the question becomes, how will this […]

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Analyze Immunostained Slides with Semiquantitative Scoring

A  routine task in the lab is to investigate the presence of your favorite protein in a range of histological samples. No doubt, staining your tissue sections using good old immunohistochemistry (IHC) would be your first choice. You just got to love a technique that has celebrated its 70th birthday, and is still used in […]

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