David Galbraith's Profile

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Sorting Large Cells and Materials by Flow Cytometry

Flow cytometers and cell sorters were designed with blood cells in mind. This means that commercial cell sorters are optimized for sorting cells typically smaller than about 20 µm in diameter. However, it turns out that many cell types, including those of mammals, are larger than 20 µm. So what are your options if you […]

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In Flow Cytometry 9th of July, 2016
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Publish and Perish: Post-Publication Review and Why YOU Should Get Involved

“13 Photoshop Fails You Won’t Believe Really Happened!”  A familiar tag-line on the internet, and you yet again waste a few minutes as you click through a series of impossible images of the human form. The word Photoshop, as for Xerox, has lost its Trademark status through the compliment of being incorporated into everyday English: […]

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Crap in, crap out: Flushing Out The Problems in Your Flow Cytometry Data

“What Have You Done To My Cells??!!!” This cry of pain from researchers, frequently aimed at core facility operators, is heard after receiving incomprehensible data for an invaluable tube of cells. Equally baffling to the trained user of flow cytometric instrumentation is when data emerges that is either unreliable or inconsistent with the known properties […]

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In Flow Cytometry 9th of September, 2014
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Sorting Single Cells – What Do You Need to Consider?

Flow cytometer and cell sorter manufacturers have invested considerable resources to design instruments that are the “fastest in the ‘hood” either in terms of cells analyzed per second, or in total throughput. The general idea is the faster you can go, the quicker you can identify rare cells, and produce sorted populations containing large numbers […]

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In Flow Cytometry 8th of July, 2014