Chelsey Kline

Articles by Chelsey Kline:

The Night Shift: Experiences at a National Synchrotron

Pulling an over-nighter is a common theme for many of my college friends. I, however, like my sleep—particularly between midnight and 7 am. Therefore, I tend not to stay up late to study and avoided jobs that required me to work a night shift. Then, I went to grad school and had to put in…

10 Jul 2017 Survive & Thrive

Advice for Working with Anaerobic Chambers

Working with anaerobic chambers is a unique skill set to have. It is only necessary if you are working with oxygen sensitive compounds. For example, some metallo-proteins require an oxygen free environment to stay in a reduced state, while others are sensitive and even reactive to oxygen. Sometimes working in anaerobic chambers requires a long…

07 Jun 2017 Equipment Mastery & Hacks

Transfection Toolkit

Engineering a mutation or overexpressing a recombinant protein to study and characterize its function in mammalian cells is no easy task. Luckily, Chinese hamster ovary (CHO) cells, which have been a mainstay in the lab since the 1950s, represent a relatively easy mammalian model system to engineer. There are several methods to choose choose from…

24 May 2017 DNA / RNA Manipulation and Analysis

Don’t Let Bubbles Burst Your Experimental Excitement

Bubbles isn’t just the name of my favorite cartoon character from Power Puff girls, or just the best activity for a kid to play with, in general. In my adult world, they stand for a whole lot more, but can still cause extreme emotions. At the lab bench, seeing bubbles brings happiness or sadness depending…

22 May 2017 Basic Lab Skills & Know-how

Murphy’s Law Lives in my Lab

Some days are good and things run like clockwork. Then there are those other days – you know the ones – where you mutter to yourself, “Can’t anything go right today?!?!?” Those are the days you are subject to Murphy’s Law. And I am sorry to tell you “no, nothing will go right” those days…

01 Mar 2017 Lab Safety

Water your choices? Understanding Types of Water in the Lab

If you are working in a scientific laboratory, it is very important to be aware of the various types of water available, because the purity may not be acceptable for your specific experimental application. In most labs, there are generally two types of water piped in to the sinks: Industrial Water Industrial water is non-potable…

08 Feb 2017 Basic Lab Skills & Know-how

Scientific Conferences: What to Expect Other Than Lectures and Coffee?

Ever wanted to go to a conference, but don’t know what to expect, which one to go to, or how to even begin to ask your boss to let you attend a conference? Look no further! I have some suggestions on how to choose from the many great scientific conferences out there, how to get…

14 Dec 2016 Career Development & Networking

Working in a Cold Room Without a Parka?

Have you ever needed to work in a cold room for a long period of time? For example, if you need to dialyze or purify a protein of interest that is temperature sensitive, working in a 4°C cold room might be the only way to accomplish the work. Well, you are in luck. I dislike…

10 Oct 2016 Basic Lab Skills & Know-how

FTIR Spectroscopy: Every Window Needs a Good Acid Wash

Fourier Transform Infrared spectroscopy (FTIR spectroscopy) is a useful and exquisitely sensitive technique used to identify and quantify unknown compounds, as well as study fine molecular details. However, to obtain a meaningful IR spectrum, it is not only important to prepare the sample correctly but also to learn how to clean the apparatus that houses…

27 Sep 2016 Analytical Chemistry and Chromatography Techniques

Harvest Large Quantities of Secreted Protein with Hollow Fiber Bioreactors

Mammalian cell culture techniques are not simple, and culturing the cells requires a lot of maintenance as well as patience. In addition, doubling times compared to bacterial cells can take days instead of hours, which is most evident when contamination occurs. However, implementing small-scale hollow fiber bioreactors for culturing mammalian cells can save a lot…

09 Jul 2016 Cells and Model Organisms