Christopher Dieni's Profile

enzymes, label free decetion read on

Working with Enzymes: Part I -The Simple Kinetic Spectrophotometric Assay

At the end of my last article, I provided some practical tips and tricks for working with enzymes at the bench. Now, we’ll cover one of the cornerstone techniques of enzymology work: the enzyme assay. Starting with the simple assays and eventually working our way to the more complex, this article introduces the principles of […]

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Working with Enzymes: Stability, Purification and Activity

In Part 1 of this series, we began our journey into the fascinating world of enzymology. We looked at the most basic concepts of what an enzyme is and the incredible jobs it can do. In Part 2 of “Working with Enzymes,” I will look at some things that you should keep in mind to […]

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So You Want to Work With Enzymes: What Is An Enzyme?

Protein kinases and protein phosphatases phosphorylate and dephosphorylate a plethora of proteins. They are responsible for regulating the majority of cellular activities. Because of their importance, they can seem intimidating to tackle as a research project. At the end of the day however, kinases and phosphatases are- simply put- enzymes. Therefore, you can standard enzyme […]

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In Protein Expression & Analysis 7th of February, 2011
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Basics of Protein Phosphorylation Part V: Further Reading

Chris Dieni wraps up his series on the Basics of Protein Phosphorylation with this final part on further reading and resources available.

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In Protein Expression & Analysis 21st of November, 2010
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Basics of Protein Phosphorylation Part IV: Taking It Off

Chris Dieni explores the promiscuous yet intriguing nature of protein phosphatases

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In Protein Expression & Analysis 10th of November, 2010
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Basics of Protein Phosphorylation Part III: Family Ties – Diversity and Similarity Among Protein Kinases

So far in our “Basics” series, we’ve taken an overview of the major players involved in protein phosphorylation, and some of the tools that one might use to study them. Now, we’ll return to the protein kinases to take a deeper look- though our “deeper” look will barely scratch the surface, as there are currently […]

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In Protein Expression & Analysis 29th of September, 2010
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Basics of Protein Phosphorylation Part II: Tools of the Trade

In the previous article in this series, we looked at the major players involved in protein phosphorylation: protein kinases, protein phosphatases, and target proteins. This time, we’ll glance over some of the tools that we can use to study various aspects of protein phosphorylation, focussing on a few I’ve personally come across. 1. Tools for […]

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Basics of Protein Phosphorylation Part I: Introducing the Candidates

The focus of my grad studies and postdoctoral research has been the analysis of proteins regulated by reversible protein phosphorylation. However, the number of unique facets in which protein phosphorylation can be studied is astounding, and is diverse as any niche of the biosciences. This article is the first in a series that will give […]

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Where Did Bio Begin For You?

Where did bio begin for you? That intriguing question has been the central focus of an interesting experience I just had. How this all started It began on a Saturday morning. After I woke up and fed my cat, and before making my own breakfast, I went straight to my computer to check which emails […]

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In Software & Online Tools 5th of February, 2010