Cindy Duarte Castelão

Cindy received a Biomedical degree in 2011, and a MsC in Molecular Biology and Genetics in 2011. Her career so far has been in the Molecular Genetics field, working closely with deep-sequencing techniques, and how these techniques can help to unveil the secrets of the genome and epigenome. Join me in uncovering the mystery. Publications by Cindy Duarte Castelão

Articles by Cindy Duarte Castelão:

An Introduction to Nanopore Sequencing

DNA sequencing is the most powerful method to reveal genetic variations at the molecular level, leading to a better understanding of our body in physiological settings, and pathological conditions. It is the beginning of the long road towards better diagnostics and personalized medicine. Even though there have been great advances in DNA sequencing technologies there…

18 Jul 2017 Genomics & Epigenetics

Oligo Purification Methods: How, Why and for What?

Who amongst us hasn’t had the need for oligonucleotides in an experiment? It is a cornerstone in many procedures and techniques. Depending on the goal, it can be very hard to design just the right oligo for your experiment.  Oligos must have the right length; the right amount of C-G, T-A; they can’t form secondary…

18 Jul 2017 PCR, qPCR and qRT-PCR

Demystifying NGS: Depth Coverage and Deep Sequencing

NGS is not a three-headed monster. However, it can be a difficult concept to grasp—especially when you are getting started.  There is a lot of new terminology, and a whole new world to discover: both in the lab bench and in interpreting your results. It helps to start somewhere. So, let’s start! Depth of Coverage…

18 Apr 2017 Genomics & Epigenetics

How to Focus When Your Colleagues Are Wreaking Havoc Around You

Now, we all love our colleagues – true, some are more loveable than others, but still. However, sometimes they can be very noisy. Especially when you need to focus.  For example, when you are in the middle of some important breakthrough, or trying to decipher a cryptic, but important paper. If you work in a…

29 Mar 2017 Dealing with Fellow Scientists

Variations on the ChIP-seq Theme and Challenges of Befriending Large Datasets

ChIP-seq has proved amazing. Through these new techniques, we can obtain big datasets in a matter of days, making our lives in the lab easier and more efficient. ChiP-seq combines chromatin immunoprecipitation (ChIP) assays with whole genome sequencing. This makes it possible to understand where proteins bind to DNA and epigenetic modifications. Humans are not only their…

31 Jan 2017 Genomics & Epigenetics

7 Tips for using Magnetic Beads for DNA Cleanup

Whatever molecular biology techniques you use, at some point you will have to clean up your DNA samples to remove things like buffers, contaminants and nucleotides from you precious sample, so that you have perfectly pure DNA for your downstream experiments. Magnetic beads are one DNA cleanup option. They are simple and effective—and their reassuringly…

03 Nov 2016 DNA / RNA Manipulation and Analysis

How to Fit in With a New Laboratory Group

Change is never easy, especially when you are trying to fit in with a new laboratory group. Here are some tips to make your transition bearable. Or even pleasant.

09 Jul 2016 Survive & Thrive

How to Choose a PhD Topic

Choosing a PhD topic can be very hard. There are a lot of things to consider from the subject to the supervisor. Here are some tips to help you choose. Find out what you really like This is the first topic because it is the most important. My first advice would be to get some…

09 Jul 2016 Career Development & Networking&Lab Statistics & Math

Want NGS Success? Consider These Three Factors

NGS is still something a little scary for most operators. Mainly because of the price, which can make the pipette, in the hand of the best of us, shake a little with fear. And even though the protocols have a tendency for getting simpler, faster, and more appropriate for a routine, there are still crucial…

09 Jul 2016 Genomics & Epigenetics