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Challenges of Autofluorescence in Neuroscience

If you have ever imaged biological samples, you have likely encountered autofluorescence. That pesky background coloration you see under the microscope, which can make it difficult to distinguish your actual signal from the noise.1 When you are trying to look for something as delicate as RNA, you don’t want to be hunting for your signal […]

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In Microscopy & Imaging 30th of November, 2016
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Quenching to Success with BHQnova Probes: Turn to the Dark Side

The ability to specifically detect and quantify the amount of a particular nucleic acid sequence in a sample has revolutionized molecular biology and science as a whole. We can now elucidate many genetic elements, such as identify genomic copy number variations, the presence of pathogens, or assess the expression of a gene in large populations. […]

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Genotyping Two Ways: Introducing BHQplus® Probes and KASP™ Assays for SNP Detection

The internet and affordable airfare have made the world a considerably smaller place. This trend is reflected in how we study diseases and science in general. International collaborations and worldwide consortia have been established to further scientific knowledge (and not just for the free trips!). This shift towards greater collaborative efforts is particularly evident in […]

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Detecting Signal in qPCR: From DNA Binding Dyes to BHQ Probes

A Brief History of Detecting Amplicons The Old Days of Ethidium Bromide In the early 1990s, quantitative (q) PCR was in its infancy, and despite PCR itself already being around for 10 years, there were no easy ways of precisely quantifying the amount of DNA that was amplified in a PCR reaction. In those days PCR […]

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FISHing Made Easy with Stellaris® DesignReady Assays from Biosearch Technologies

Do you want to directly quantify RNA? Do you want to be able to localize RNA simply, quickly, and precisely? Heard about RNA FISH but are not sure how to go about designing your own probes? Then you want Biosearch Technologies’ DesignReady assays for Stellaris RNA. We’ve already seen the power of Stellaris® in our […]

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Modify Your Oligos, Modify Your Experiments

If you’ve ever performed PCR, you’re probably already very familiar with DNA oligonucleotides (or oligos). But did you know that these molecules can do so much more than just act as simple primers? You can add a wide range of modifications to your oligos, which may change the stability, binding, solubility and even visibility, to […]

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How RNA-FISH Can Complement qPCR, and Vice Versa

In studies of RNA abundance and gene expression, no one technique can answer all of the questions that need to be asked. So it is necessary to use a variety of experimental methods in concert. Two RNA detection and measurement techniques that complement each other well for this purpose are RNA Fluorescence in situ hybridization […]

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A primer on Probe-based SNP genotyping

Single Nucleotide polymorphisms (SNPs), colloquially pronounced ‘snips’, are the most common type of genetic variation in people. By definition, a SNP represents a single nucleotide variation at a specific location in the genome that is found in more than 1% in the population. For example, a SNP can replace the nucleotide cytosine (C) with an […]

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In PCR, qPCR and qRT-PCR 13th of December, 2013
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A Quick Tour Around Probe-based Multiplexing qPCR

PCR has become the tool of choice for molecular diagnostics and is now a staple platform in any laboratory setting. The versatility of this method has led to a myriad of spin-off techniques, including probe-based quantitative PCR (qPCR). This method effectively combines PCR amplification and detection into a single step to measure the specific amount […]

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In PCR, qPCR and qRT-PCR 29th of November, 2013
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PCR is 30! Here’s How it has Rocked the World

This year marks an important birthday for molecular biologists: the polymerase chain reaction (PCR) turns 30!  This is my eulogy to celebrate life so far for the technique that has rocked the world. PCR has become such an integral part of the bioscientist’s toolbox that new generations of researchers probably cannot imagine life without it. It […]

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In PCR, qPCR and qRT-PCR 15th of November, 2013