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How to Choose Your Method for DNA Extraction from Whole Blood

The technique you choose for whole blood DNA extraction impacts not only your results but also the ease of your workflow. It can be difficult to choose which method to use. Almost all of the available protocols for whole blood DNA isolation involve these common steps: cell lysis, removal of protein, other contaminants and RNA, […]

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In DNA / RNA Manipulation and Analysis 20th of September, 2016
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Eight Top Tips to Maximize Yield from Whole Blood DNA Isolation

When you perform genomic DNA extraction from whole blood, low yield or low quality DNA can result in many issues. No matter your intended downstream application—qPCR, next generation sequencing, Sanger sequencing, and so on—you need high quality DNA. We’ve made this step-by-step guide to assist you in getting the highest possible DNA yield and quality, and […]

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In DNA / RNA Manipulation and Analysis 20th of September, 2016
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Choosing The Right Blood Collection Tubes

So you’re designing a project using human blood samples. Maybe you’re studying blood cells, maybe you’re after genetic material or circulating biomarkers. For many basic scientists, the process of getting that blood out of a person and into your project can be intimidating. Where Do You Start? Whether you’re collecting your samples in-house or through […]

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In Chemistry for Biologists 4th of May, 2015
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Clean Up Your Act! How To Clean Up PCR Contamination

You carefully set up your PCR, excitedly waited for it to finish, ran your gel, and waited for the big reveal. But instead of seeing what you hoped (a nice clean gel), you see a big fat mess—extra bands and, most disturbingly, bands in your negative control. So, what are you to do? How do […]

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In PCR, qPCR and qRT-PCR 1st of January, 2015
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A Beginner’s Guide to Next Generation Sequencing (NGS) Technology

The completion of the Human Genome Project in 2003 ushered in a new era of rapid, affordable, and accurate genome analysis—called Next Generation Sequencing (NGS). NGS builds upon “first generation sequencing” technologies to yield accurate and cost-effective sequencing results. Fred Sanger sequenced the first whole DNA genome, the virus phage φX174, in 1977. In that […]

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In Genomics & Epigenetics 2nd of October, 2014