Aunt Yersinia's Profile

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How To Ask For Reagents

Greetings, I’m a research student and I’m having a lot of trouble in finding the right antibodies for my study, because I need both monoclonal and polyclonal. And I need to start addressing some other international scientists. The problem is that I don’t know how to address the situation. If they indeed have antibodies that […]

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In Aunt Yersinia 9th of July, 2016
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Am I Damaging My E. coli by Spinning at High Speeds?

Dear Aunt Yersinia, A very annoying postdoc in our group keeps telling me off for spinning E.coli at 13K in a tabletop centrifuge. The postdoc claims that high speed damages cytoskeleton and this will reduce my transformation frequency. But I don’t believe her as the cells are cushioned by water during centrifugation. Can you tell […]

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In Aunt Yersinia 18th of March, 2015
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Help: My Gal Promoter Doesn’t Work

Dear Aunt Yersinia, I work with a yeast vector where my gene is under a Gal promoter. My boss told me to grow yeast in medium with sucrose, and then add galactose to induce the promoter. I did it, but my protein is not induced; the Western blot is empty. I sequenced a part of […]

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In Aunt Yersinia 18th of August, 2014
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To Pour or to Spread, This is the Question

Dear Aunt Yersinia, What are advantages and disadvantages of pour and spread plates? Antonio   It is refreshing to see such wisdom – knowing that there are at least two methods of pouring plates – in a young man. While all molecular biologists know or have at least seen a ”spread plate” aka Petri dish […]

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In Aunt Yersinia 9th of July, 2014
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Microbiology 101 by Aunt Yersinia

We are pleased to announce that the famous maid of microbiology, dear old Aunt Yersinia, has agreed to start writing a microbiology and molecular biology advice column for Bitesize Bio. She will be free to answer your most pressing questions sent to:[email protected] By way of introduction, Aunt Yersinia is bestowing 8 spores of knowledge garnered […]

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In Microbiology 21st of May, 2014