Alex Reis's Profile

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Should PCR cloning be a part of your molecular cloning toolkit?

While the classic approach to molecular cloning – using restriction enzymes to excise a DNA fragment of interest – is as useful as ever, new techniques that make cloning faster, easier and more versatile are available. As a smart molecular biologist, you should be examining each of them to see whether or not adding them […]

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When Gibson Assembly is the best option: a case study with P. falciparum

Malaria continues to be a leading case of mortality worldwide. It is estimated that the parasitic disease, carried by infected mosquitoes, spread to over 200 million cases in 2010, resulting in 660,000 deaths. No vaccine yet Unfortunately there’s no vaccine yet! For the time being, treatment relies heavily on the use of a limited number […]

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In DNA / RNA Manipulation and Analysis 26th of February, 2014
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The Future of PCR

After 30 years, what’s next for PCR? In the 80s, saying you were doing “PCR” was enough for everybody to know what kind of experiment you were performing. Today, the “PCR family” has gained so many new members that it can be confusing to decipher which type of PCR you’re using. Developed in 1983 by […]

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In PCR, qPCR and qRT-PCR 17th of February, 2014
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10 ways to improve blunt-end ligations

Blunt-end cloning involves the ligation of DNA fragments – usually between a plasmid vector and an insert – whose terminal ends are not “sticky”. Performing these ligations is notoriously difficult, particularly with large DNA fragments. But it is possible. And in this article I’ll give you some tips that I hope will increase your chances […]

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In DNA / RNA Manipulation and Analysis 20th of January, 2014
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Choosing the right plasmid vector: A Guide for beginners

If you’re picking up a list of available plasmid vectors for the first time, it can be mind-blowing to decide on the best one for your experiment. What cloning sites do you need? What restriction enzyme sites? What insert size is possible? Let me help you with some pointers about what factors to look out […]

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In DNA / RNA Manipulation and Analysis 13th of January, 2014
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Breaking The Code Of The Mummies With NGS: A Hallowe’en Special!

Ancient Egyptians believed in the afterlife. For them, death was merely a passage from a temporary body into a permanent spiritual existence. However, the preservation of the physical body was crucial for a happy eternity. The answer: artificial mummification. It’s a secret For most of us, the word ‘mummy’ immediately brings to mind images of […]

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In Genomics & Epigenetics 31st of October, 2013