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Making the Most of Quiet Days in the Lab: From Gloomy to Glorious

It's Monday morning. You arrive in the lab armed with a large coffee and feeling rested after a non-lab weekend. You check your email and calendar and peek into your PI's office. Today will be a rare...
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Writing a Successful Graduate Fellowship Application

The most obvious benefit of a graduate fellowship is the generous monetary support that pays for your stipend, tuition, and/or travel expenses. The not-so-obvious benefits of writing a graduate fellowship...
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Article

Demystifying the Flow Cytometry Optics System: A Peek Under the Hood

To many users, the flow cytometer is a magic box: put in cells, get out data. You click the button to tell it which colors to look at without much thought about how the machine does this. However, not all...
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Article

How to Keep Your Centrifuge Alive

According to the Occupational Safety and Health Administration, "the majority of all centrifuge accidents result from user error." That's right, it's us. Many a machine has been destroyed by an unsuspecting...
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Article

Acid Phenol Chloroform Extraction of DNA, RNA and protein: 3 in 1

In austerity times, nothing is in excess. Apart from saving reagents, which can be refilled with extra financial injections, there is a commodity that cannot be easily resupplied – tissue samples! If, like...
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Article

Pack a Chromatography Column Like a Pro

You already learned the basics of column packing. When moving to more automated system using low pressure liquid chromatography systems, you can use pre-packed columns. But in order to compare several resins in...
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Article

Are you Guilty of P-Hacking?

P-value abuse directly contributes to one of the biggest problems facing the scientific community: the prominence of false-positive results in the published literature. Contrary to popular interpretation, the...
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Article

The Obligate qPCR Standard Curve

At first sight, real-time PCR looks like a very simple technique—very straightforward. Also, when it's optimized, real-time PCR leads to interesting results. However, to obtain consistent and accurate...
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Article

Four Tips for Working with Human Clinical Samples

While using human clinical samples in your research can provide robust and heterogeneous results applicable to larger portions of the population, working with these samples presents its own set of...
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Article

How to Avoid Feeling Isolated During Graduate School

Try to recall the last time you were not alone in the lab at 11pm on a Friday night running an experiment. If you found that to be difficult, then this article is definitely for you. Here are ways to avoid...
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