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Low cost DNA gel documentation

Equipment for photographing DNA gels stained with ethidium bromide (or other fluorescent dyes), doesn't have to cost thousands of dollars. These days, great pictures can be obtained with a standard digital...
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My favorite PCR polymerase

What do you get when you cross a Pyrococcus DNA polymerase with a dsDNA binding domain? ...a highly processive, high fidelity, lighting fast PCR work-horseThose Finnish wizards at Finnzymes have made that...
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Plasmid Cut & Paste with Quikchange

I recently had a problem where I needed to cut out an expression cassette (a promoter coupled to a coding sequence) from one plasmid vector and paste it into another containing an expression cassette so I...
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Zyppy Plasmid Miniprep Kit

Zymo research have launched a new plasmid miniprep kit that not only is has the coolest name of any kit on the market but is, the manufacturers claim, the fastest means of plasmid purification available. In...
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RNA Polymerase II Assayed in Living Cells

A Nature Structural & Molecular Biology article published by Singer et al has provided a fascinating insight into the kinetics of RNA polymerase II during transcription. Using a combination of extremely...
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