The Sandwich Technique: How to Dish Out Critique

By Vicki Doronina | July 19, 2017

How time flies. One day you are giving your first presentation in front of your fellow students, seemingly the next you are listening to a presentation by your own student. And frankly, the talk is awful. It’s twice the time limit, the student weaves around the topic as a drunken sailor, and she acknowledges her…

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Oligo Purification Methods: How, Why and for What?

By Cindy Duarte Castelão | July 18, 2017

Who amongst us hasn’t had the need for oligonucleotides in an experiment? It is a cornerstone in many procedures and techniques. Depending on the goal, it can be very hard to design just the right oligo for your experiment.  Oligos must have the right length; the right amount of C-G, T-A; they can’t form secondary…

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An Introduction to Nanopore Sequencing

By Cindy Duarte Castelão | July 18, 2017

DNA sequencing is the most powerful method to reveal genetic variations at the molecular level, leading to a better understanding of our body in physiological settings, and pathological conditions. It is the beginning of the long road towards better diagnostics and personalized medicine. Even though there have been great advances in DNA sequencing technologies there…

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Can’t Decide Between Medical School or Graduate School?

By Ryan Heck | July 17, 2017

For the longest time I just couldn’t decide between medical school and graduate school. I mean come on, being a physician would be great. I could save lives just like they do on TV! But on the other hand, being a biomedical scientist excited me just as much. You can’t tell me you’ve never dreamt…

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Tips for Getting Your Neurons Firing… Consistently

By Manisha Sinha | July 13, 2017

Primary cultures of rodent (rat and mice) neurons are widely used for disease modeling and studying cellular mechanisms in neurobiology. If you are in this field and need help with protocols and batch-to-batch variability of your dissociated primary rodent neurons, read further below. Also consider watching several JoVE (Journal of Visualized Experiments) articles.1-24 These cover…

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Guidelines for Efficient Cell Sorting – Part 1

By Vaidyanathan Subramaniam | July 11, 2017

Flow cytometry is a pervasive tool to characterize just about anything in cell biology. From quantifying the expression of surface antigens, to determining the physiological changes in cells and everything in between, flow cytometry is as indispensable to a cell biologist as a knife is to a surgeon. Cell sorting is pivotal in enabling researchers…

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Brefeldin A v Monensin: How to Hunt for Proteins

By Kristen Haberthur | July 11, 2017

As any good biologist knows, one of the easiest ways to determine if a cell is functionally active is the production and secretion of proteins in response to a stimulus. In many circumstances, the quantity of the secreted protein, and thus the level of cellular activation can be assessed by ELISA. However, if you are…

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Using Synthetic DNA For Long Term Data Storage

By Alex Chen | July 10, 2017

The amount of data requiring long-term storage is growing and accelerating. Current long-term digital storage technology cannot keep up. Imagine roughly 2.5 QUINTILLION bytes of data being created everyday in this world1–2 as more computers and network infrastructure come online. For average users, a long-term storage solution is probably not an issue. However, organizations and…

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The Night Shift: Experiences at a National Synchrotron

By Chelsey Kline | July 10, 2017

Pulling an over-nighter is a common theme for many of my college friends. I, however, like my sleep—particularly between midnight and 7 am. Therefore, I tend not to stay up late to study and avoided jobs that required me to work a night shift. Then, I went to grad school and had to put in…

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Taking up the Challenges of In Vitro Monoclonal Antibody Production

By QED Bioscience | July 7, 2017

Monoclonal antibodies are extensively used in research laboratories, diagnostic products and immunotherapy and have multiple advantages over polyclonal antibodies. They exhibit enhanced specificity to single epitopes, have little or no variability, and are easy to modify and customize as required. The History of Monoclonal Antibodies In 1984, Georges Köhler, César Milstein, and Niels Jerne received…

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