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You Know You’ve Been In the Lab Too Long When…

This has been doing the rounds all over the web, so I thought I'd post my 10 favorites. You know you've worked in the lab too long when... You wash your hands before you go to the toilet You tell your...
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Ethidium Bromide: A Reality Check

The hysteria among molecular biologists about our old friend ethidium bromide has long been an irritation to me. Researchers are rightly wary of this potential carcinogen. More recently this wariness has been...
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Protein Expression with a Cherry on Top

If you do a lot of heterologous protein expression, take a look at Eurogentec's CherryExpress Kit. Based on the T7 expression system, the CherryExpress vector has a sequence encoding a small red polypeptide...
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Choosing a Competent E.coli Strain

Of all the of competent E. coli cell strains available, which one should you choose? The choice of strain to use in a given experiment is determined in large part by the nature of the experiment and the set of...
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The World’s Fastest Miniprep?

I love this great time-lapse video entitled "a day in the lab". If only minipreps were this fast and easy! [youtube:http://www.youtube.com/watch?v=XKF_bItwkjk]
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Competent E.coli: To buy or not to buy?

Buying competent cells from commercial suppliers is convenient, provides a guarantee of quality and gives access to strains with a variety of in-built traits that assist with things like maintenance of...
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15 Reasons to Be a Scientist

Just for fun, here my top 15 reasons for being a scientist. Add your own reasons in the comments below if you so wish. 1. Not being stuck behind a desk all day every day 2. Conferences... see the world...
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One Tube PCR Cloning Method

I love shortcuts, and this one is very good. Chun-Ming Liu of Plant Research International has a number of molecular biology protocols on his website,  but my favourite is his One Tube PCR Cloning Method. The...
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Binder CO2 incubator – movie fun

Binder have released a fun movie entitled "Germfellas" to promote their latest CO2 incubator, which comes with inbuilt 180°C sterilization capability. The movie is nicely done and is a great piece of...
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10 Tips for Better Presentations

I have been at a conference today and don't have too much time to write this, so this will be a quick article. After watching lots of speakers of varying competence, I thought that it would be good to outline...
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