Techniques / Protein Expression & Analysis

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When SDS-PAGE Goes Bad

You are not alone. Everyone makes a hash of their protein gel sometimes but this resource can help you work out what went wrong, and feel better for seeing gels even worse than yours.
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Southern, northern, western (and eastern?)

This is the story of how one of the most famous and quirky naming conventions in biology came into being.
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Article

Folding can be fun

If you've been interested in the Folding at Home project, but wanted to play a much more active role then your wait is over.
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How to Refold 653 Insoluble Proteins

An introduction to the Monash protein folding database, which contains protocols for refolding over 600 different proteins from inclusion bodies.
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Lazy Cell Lysis

For routine procedures involving cell lysis, it's good for the lysis to be... routine. Of course there are many good and freely available lysis buffer recipes but for convenience and reproducibility you can't...
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An Easy Lysis / Homogenization Method for Any Sample

Lysis of some microbes is easy, but for others its much more difficult - I think due to differences in the make-up of their cell wall. At the moment, one of my colleagues is preparing hundreds of cell-free...
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Enzyme Commission (EC) Numbers

In the early 1950's so many new enzymes were being discovered in the burgeoning field of biochemistry that enzyme nomenclature was in danger of getting out of hand. With no guidelines on how to name enzymes,...
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Article

A Missing Post-translational Modification

Eukaryotic cells possess a surveillance mechanisms that identifies aberrantly processed mRNA precursors and prevents their flow to the cytoplasm by tethering them near the site of transcription. Termed...
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Protein Expression with a Cherry on Top

If you do a lot of heterologous protein expression, take a look at Eurogentec's CherryExpress Kit. Based on the T7 expression system, the CherryExpress vector has a sequence encoding a small red polypeptide...
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