Techniques / Protein Expression & Analysis

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Super Fast Immunoblotting

Sometimes a new product comes along and you just know that it is going to make your life easier. The SNAP i.d. protein detection system from Millipore falls into that category. In a nutshell, SNAP i.d. is a...
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An Intro to Cell-free Protein synthesis

Cell-free protein synthesis (aka In vitro translation) refers to protein production in vitro using lysates generated that provide the cellular machinery necessary for synthesis. The lysates can be of bacterial...
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What you need to know about OD600

If you use a spec to measure cell density, you may be making a very common mistake and taking inaccurate measurements as a result. Specs are often used for measuring the density of suspension cultures, but...
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When SDS-PAGE Goes Bad

You are not alone. Everyone makes a hash of their protein gel sometimes but this resource can help you work out what went wrong, and feel better for seeing gels even worse than yours.
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Southern, northern, western (and eastern?)

This is the story of how one of the most famous and quirky naming conventions in biology came into being.
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Folding can be fun

If you've been interested in the Folding at Home project, but wanted to play a much more active role then your wait is over.
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How to Refold 653 Insoluble Proteins

An introduction to the Monash protein folding database, which contains protocols for refolding over 600 different proteins from inclusion bodies.
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Lazy Cell Lysis

For routine procedures involving cell lysis, it's good for the lysis to be... routine. Of course there are many good and freely available lysis buffer recipes but for convenience and reproducibility you can't...
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An Easy Lysis / Homogenization Method for Any Sample

Lysis of some microbes is easy, but for others its much more difficult - I think due to differences in the make-up of their cell wall. At the moment, one of my colleagues is preparing hundreds of cell-free...
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Enzyme Commission (EC) Numbers

In the early 1950's so many new enzymes were being discovered in the burgeoning field of biochemistry that enzyme nomenclature was in danger of getting out of hand. With no guidelines on how to name enzymes,...
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