Protein Expression & Analysis

DNA Jelly for Cell-Free Protein Synthesis

I had previously talked about the basics of cell-free protein synthesis and ways to improve yields from the technique. But recently I came across an article describing a technology that promises to dramatically improve the efficiency of cell free protein synthesis. The article, published in Nature Materials, shows a novel way to produce proteins cell-free…

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Solved:Low Yields in Cell-Free protein Synthesis

In my last article, I introduced Cell-Free Protein Synthesis. Today I want to talk about a major bottleneck in in vitro cell-free protein expression; low yield. Most often, paying attention to the important factors such as template purity & design, transcription and translation inhibitors and potassium and magnesium concentration will solve any problems with low…

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Super Fast Immunoblotting

Sometimes a new product comes along and you just know that it is going to make your life easier. The SNAP i.d. protein detection system from Millipore falls into that category. In a nutshell, SNAP i.d. is a nifty little gadget that fits in to the immunoblotting protocol after the membrane transfer step. There it…

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An Intro to Cell-free Protein synthesis

Cell-free protein synthesis (aka In vitro translation) refers to protein production in vitro using lysates generated that provide the cellular machinery necessary for synthesis. The lysates can be of bacterial or eukaryotic origin. It is a useful alternative to in vivo synthesis for generating protein for the study of things like: protein:protein interactions (pull-down assays,…

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