Techniques / Protein Expression & Analysis

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SDS-PAGE: The Easy Way to Find the Wells

If you have ever attempted to load a SDS-PAGE gel only to miss the well, stab the divider, and then watch helplessly as your sample squirts off into the wrong well, then this tip is for you. The fortunate...
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New Magnetic Particle Processor Software for the Kingfisher

Thermo Fisher Scientific Inc., the world leader in serving science, today announced the new Thermo Scientific BindIt 3.1 software for use with all Thermo Scientific KingFisher magnetic particle processor...
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In Protein Expression & Analysis
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New Downloadable Protocols Available for the Multiskan FC Microplate Photometer

Thermo Fisher Scientific Inc., the world leader in serving science, today announced a new range of downloadable protocols for the Thermo Scientific Multiskan FC microplate photometer. There are now nearly 50...
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In Protein Expression & Analysis
Article

DNA Jelly for Cell-Free Protein Synthesis

I had previously talked about the basics of cell-free protein synthesis and ways to improve yields from the technique. But recently I came across an article describing a technology that promises to dramatically...
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Article

Solved:Low Yields in Cell-Free protein Synthesis

In my last article, I introduced Cell-Free Protein Synthesis. Today I want to talk about a major bottleneck in in vitro cell-free protein expression; low yield. Most often, paying attention to the...
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Super Fast Immunoblotting

Sometimes a new product comes along and you just know that it is going to make your life easier. The SNAP i.d. protein detection system from Millipore falls into that category. In a nutshell, SNAP i.d. is a...
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Article

An Intro to Cell-free Protein synthesis

Cell-free protein synthesis (aka In vitro translation) refers to protein production in vitro using lysates generated that provide the cellular machinery necessary for synthesis. The lysates can be of bacterial...
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What you need to know about OD600

If you use a spec to measure cell density, you may be making a very common mistake and taking inaccurate measurements as a result. Specs are often used for measuring the density of suspension cultures, but...
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When SDS-PAGE Goes Bad

You are not alone. Everyone makes a hash of their protein gel sometimes but this resource can help you work out what went wrong, and feel better for seeing gels even worse than yours.
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Southern, northern, western (and eastern?)

This is the story of how one of the most famous and quirky naming conventions in biology came into being.
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