Protein Expression & Analysis

Lazy Cell Lysis

For routine procedures involving cell lysis, it’s good for the lysis to be… routine. Of course there are many good and freely available lysis buffer recipes but for convenience and reproducibility you can’t beat pre-made lysis buffers. Focusing on lysis for protein extraction, here are some of the reagents available for fast and efficient lysis…

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Enzyme Commission (EC) Numbers

In the early 1950’s so many new enzymes were being discovered in the burgeoning field of biochemistry that enzyme nomenclature was in danger of getting out of hand. With no guidelines on how to name enzymes, researchers simply chose their own. Some enzymes were given names, like diaphorase or Zwischenferment, that conveyed nothing about the…

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A Missing Post-translational Modification

Eukaryotic cells possess a surveillance mechanisms that identifies aberrantly processed mRNA precursors and prevents their flow to the cytoplasm by tethering them near the site of transcription. Termed post-translational modification, this process includes the distinct events of 5′ capping, 3′ polyadenylation, and intron splicing. During processing, nascent mRNA assembles together with RNA binding proteins into…

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Protein Expression with a Cherry on Top

If you do a lot of heterologous protein expression, take a look at Eurogentec’s CherryExpress Kit. Based on the T7 expression system, the CherryExpress vector has a sequence encoding a small red polypeptide (the heme binding part of cytochrome) fused to the promoter. When your favorite gene is cloned in, the resulting fusion protein is…

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