Techniques / PCR, qPCR and qRT-PCR

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Article

No More White Elephants! – Consider this Before Buying a Real-time PCR Cycler

Does your lab have a closet full of white elephants; once expensive instruments that are no longer fit for purpose, or have broken down? In many cases, all of that wasted money and resource could have been...
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Article

When PCR Gets RACE-y: From Unknown mRNA Segments to Sequenced cDNA

Normally you need two primers to amplify your segment of interest - one for the 3' end of your segment of interest and one for your 5' end. But if you don't know the sequence of the regions you're hoping to...
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Article

How to Quantify Integrated HIV Genomes Using Alu-gag PCR

Alu sequences are repetitive DNA sequences that are widely dispersed within the human genome. These “junk DNAs” are not as useless as one might think. An interesting method to use them is to quantify the number of integrated Human Immunodeficiency Virus (HIV) genome copies using Alu-PCR.
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Article

Entering the Digital Age for Quantitative PCR Analysis: Digital PCR

Digital PCR (dPCR) is a next generation qPCR that you might just need for quantitation and comparison of minute genetic copy differences.
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Article

10 Tips for Consistent Real-Time PCR

Real-time PCR is a specialized technique that delivers far more information about your DNA or RNA than end-point PCR. Essentially, real-time PCR is a way to visualize the amplification of specific DNA...
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Article

Do You Know Where You Are? Find Out with Genome Walking

Do you work with plants? Are they genetically engineered? Do you know where and how? If not, you could experience problems. After all you do not want your transgenic gene cassette to disrupt genes that would...
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Article

2015 Staff Picks: PCR & Real-time PCR

To wrap up 2015, we’re highlighting our favorite articles from the year. These articles were handpicked by our editorial staff here at Bitesize Bio. So, sit back with your cup of coffee (or beverage of...
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Article

Time to Instigate Nested PCR

How to Obtain a Purer PCR Product and Reduce Non-specific Amplification Unless you’ve gotten your hands on some miraculously specific primers, amplification of only your target sequence without non-specific...
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Article

Long-Range PCR: It’s About Choosing the Right Enzyme

The ability for DNA polymerase to copy a long stretch of DNA is becoming increasingly important. Why? It has to do with the advances in our sequencing technologies. Our next generation sequencing (NGS)...
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Article

Photonic PCR: When Lightening Strikes Your DNA

Before I get into today’s topic, please allow me to digress a bit and start with a few sentences that sum up the polymerase chain reaction (PCR); the grand-daddy of molecular biology. PCR, a method that is...
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