Techniques / PCR, qPCR and qRT-PCR

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Article

Fast, Accurate and Green PCR

Attending the 2009 American Society of Microbiology meeting this week in Philadelphia, I decided to take my own advice and visit the exhibits to see whether any of the exhibiting companies had any really...
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Article

A New Use for Acupuncture in qPCR

What do you get when you cross eastern medicine with molecular biology? How about a novel way to isolate mRNA from living cells using acupuncture? That's just one of many projects going on in Dr. Karl...
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Article

Quantitative RT-PCR: One-step or Two-step RT?

qRT-PCR (quantitative reverse transcription-polymerase chain reaction) is now the gold standard technique for mRNA detection and quantification, sensitive enough to enable quantification of RNA from a single...
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The PCR song

An extremely cheesy but fun promo from BioRad
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The Best Polymerases of 2008

Yawn.... The awards season is upon us once again. Overpaid, under-worked and over-ego'd celebrities get together to slap each other's backs and tell each other how great they are. But little do they know...
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The Invention of PCR

Few technical breakthroughs have changed the face of their field like the Polymerase Chain Reaction (PCR). Gene cloning, sequencing of complex genomes, DNA fingerprinting and DNA-based diagnostics are just some...
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My favorite PCR polymerase

What do you get when you cross a Pyrococcus DNA polymerase with a dsDNA binding domain? ...a highly processive, high fidelity, lighting fast PCR work-horseThose Finnish wizards at Finnzymes have made that...
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