Techniques

Article

How DNA Extraction Is Different in Plants

I know what you are thinking, everything is made of cells, so how different can DNA extractions be in plants? The answer is… sort-of different. The overall concept is the same. Cell membranes are lysed, DNA...
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Article

How to Generate High-Quality SNPs Data Sets from NGS

SNPs or single nucleotide polymorphisms are on many scientist’s wish list in experimental studies of genomic DNA sequences. Methods to detect SNPs have evolved. Now with the availability of high throughput...
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Article

Basic Bacterial Culturing Practices

Mastering basic bacterial culturing practices is a must if you are planning a career in microbiology! Growing bacteria might be one of the easiest things to do as a scientist. Also, as you’ve probably...
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Article

Investigating an Expression Quantitative Trait Locus (eQTL)

Thousands upon thousands of genetic variants are now associated with every disease and trait you can possibly think of. Such traits range from cancers to blood pressure, intelligence, height, weight… and many...
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Article

Tips for Peering into the Interior of Mice Using Intravital Microscopy

Intravital microscopy (IVM) is a newer approach for the imaging of living tissues and organs in live animals. A wide variety of organs can be imaged in rodents–eyes, kidneys, brain, lymph nodes, and gut, to...
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Article

Variations on the ChIP-seq Theme and Challenges of Befriending Large Datasets

ChIP-seq has proved amazing. Through these new techniques, we can obtain big datasets in a matter of days, making our lives in the lab easier and more efficient. ChiP-seq combines chromatin...
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Article

How Fluorescent Molecules Work: Shine Bright like a Diamond

Fluorescence is one of the most important and useful tools in a biologist’s toolbox. In biology, nearly every field, from physiology to immunology, uses fluorescent molecules (aka fluorophores) to detect...
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Data Analysis for Three-dimensional Volume Scanning Electron Microscopy

In recent years, three-dimensional (3D) scanning electron microscopy techniques have gained recognition in the biological sciences. In particular, array tomography, serial block face scanning electron...
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Article

CRISPR Technology Explained: Towards a CRISPR Genome!

Okay, if you are working in the biomedical research field and you have not heard about CRISPR technology, then you are way behind the trend curve. Don’t worry; I would like to share what I have learned about...
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Examining Cell Interactions with Surface Plasmon Resonance (SPR) and Identifying Epitopes using SPR-Mass Spectrometry (MS)

Surface plasmon resonance (SPR) offers highly efficient, label-free detection for quantifying biomolecular interactions in real-time. Two exciting SPR variants that have sprung up in recent years are SPR for...
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