Techniques

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Ligation Independent Cloning Primer Design

Since I wrote about the wonders of ligation independent cloning a while back, several people have contacted me to say they were confused about how to design primers for this application. It can be a bit...
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The Best Polymerases of 2008

Yawn.... The awards season is upon us once again. Overpaid, under-worked and over-ego'd celebrities get together to slap each other's backs and tell each other how great they are. But little do they know...
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Fast restriction digests?

In the old days, restriction digests were a great excuse for long lunch breaks. Come back 1-2 hours later and it's done. But, just to ruin our fun, Fermentas then NEB started to offer ranges of restriction...
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Preps in the Zyppy: How I Changed my DNA Miniprep, Gel Extraction and Concentrator Kits

Breaking up is never easy, especially when you've been together a long time. But when you meet the right one it's so much easier. Here's how I ditched my old miniprep kit for something new.
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Ligation Independent Cloning Protocol

A while back I wrote a post on a T4 DNA polymerase dependent ligation independent cloning method. In the comments, Max asked if anyone had a protocol. Since there does not appear to be a simplified protocol...
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Work Smarter With The Molecular Biologist’s Toolbar

To (hopefully) make the life of molecular biologists everywhere a little easier, I have put together a Molecular Biologist's tool-bar for Firefox/IE, which you can download here. It's main feature is the...
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PlasMa DNA: Free, cross-platform plasmid mapping and analysis software

If you are looking for some free plasmid manipulation and analysis software, then you need look no further than PlasMa DNA. It's packed with features, user friendly and looks great. Best of all, it is a...
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8 Approaches to Random Mutagenesis

Random mutagenesis is an incredibly powerful tool for altering the properties of enzymes. Imagine, for example, you were studying a G-protein coupled receptor (GPCR) and wanted to create a temperature-sensitive...
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Thinking Microbes

Cognition is a term frequently used in several loosely related ways to refer to a faculty for the human-like processing of information. Signal transduction networks certainly fit that bill, as the mediate...
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Personal Genomes and Modern Eugenics

As genetic screening becomes increasingly advanced and personal genomes become more commonplace, the potential for genetic engineering and modern eugenics is becoming a reality. "Designer babies" may not be...
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