Techniques

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Electroporation on a (96 well) Plate

I just came across a neat device now being offered by BioRad that may interest those of you who do a lot of electroporation of difficult-to-transfect mammalian cells, where tedious optimization of the...
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Enzyme Commission (EC) Numbers

In the early 1950's so many new enzymes were being discovered in the burgeoning field of biochemistry that enzyme nomenclature was in danger of getting out of hand. With no guidelines on how to name enzymes,...
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The Best of: Tech Tips

Bitesize Bio has gained a lot of new readers over the past few months so I thought it would be a good idea to highlight some of the articles newer readers may have missed. I'll do this periodically to make sure...
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Troubleshooting DNA Ligation Problems

In any experimental procedure, getting the controls right can save you a lot of work when things go wrong by allowing you to troubleshoot the source of the problem. DNA ligation is no different. In this article...
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Article

Population Genetics Mechanisms on a Genomic Scale

Three papers from UC Davis have appeared on the PLoS journals in the past few days that bring together population genetics and genomic sequencing to address questions of importance to evolutionary biology. ...
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Article

A Missing Post-translational Modification

Eukaryotic cells possess a surveillance mechanisms that identifies aberrantly processed mRNA precursors and prevents their flow to the cytoplasm by tethering them near the site of transcription. Termed...
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Article

History of Molecular Biology

As a freshman biology major in undergrad, I was introduced to molecular biology with the following description: Molecular biology represents the intersection of genetics, biochemistry and cell biology. Some...
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The Invention of PCR

Few technical breakthroughs have changed the face of their field like the Polymerase Chain Reaction (PCR). Gene cloning, sequencing of complex genomes, DNA fingerprinting and DNA-based diagnostics are just some...
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Solved: Heterologous Gene Expression Problems

When heterologous gene expression goes wrong it can be a real headache. Here's my checklist for the steps to take when you encounter problems with this dark art. 1. Check the construct by sequencing the...
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Plasmid archiving at Addgene

Addgene is a non-profit plasmid repository that stores and distributes plasmids for academic labs. It's great if you work in an academic lab and they happen to have your plasmid - drop them an order and get...
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