Techniques

Sponsored Education

Sequence and Ligation Independent Cloning

Could ligation independent cloning get any better? Well here is a technique that is sequence AND ligation independent.
Read On
Sponsored Education

Would you Sterilise Growth Media With A Microwave?

Several peer-reviewed articles advocate using microwaves for sterilization, but would you trust microwave-sterilized growth medium?
Read On
Sponsored Education

Re-cycling Electroporation Cuvettes

If you have ever worked out the price of an electroporation cuvette you will realise that, at several dollars each, they are worth recycling. Accounts on how amenable electroporation cuvettes are to...
Read On
Article

Recycle Those DNA Extraction Columns

You know those ridiculously priced and throw-away DNA mini, midi and maxi-prep columns? Well the good news is that you can actually re-use them if you are reasonably careful at regenerating them, with this...
Read On
Sponsored Education

5 Products That Could Make Your Lab Life Easier

Today I was browsing through the "new technologies" section on the Biocompare website. Apart from the amazing but super-expensive automation equipment that most of us unfortunately have little chance of getting...
Read On
Article

Faster, Cooler DNA gels

All over the world, molecular biologists are tragically wasting hours of their life running DNA gels using tris-based conduction buffers like TBE or TAE. These buffers are known to overheat at high voltages,...
Read On
Sponsored Education

5 More Tips for DNA Gel Extraction

Problems with DNA gel extraction can be a real show-stopper since this is such a routinely used procedure. But, even if you are having no particular problems, it's always nice to try and pick up some...
Read On
Article

Antibiotics as a Carbon Source

Here's the context: "Eighty years after Alexander Fleming discovered penicillin on a moldy culture dish, the battle against killer bugs is faltering. More and more bacteria - including insidious tuberculosis...
Read On
Article

Quick and Dirty Screening for Cloned Inserts

For identifying positive clones from a plasmid cloning procedure, the routine of performing a mini-prep and then checking the putative clones by restriction digestion is most commonly used. Of course, if you...
Read On
Sponsored Education

Lazy Cell Lysis

For routine procedures involving cell lysis, it's good for the lysis to be... routine. Of course there are many good and freely available lysis buffer recipes but for convenience and reproducibility you can't...
Read On