Techniques / Microscopy & Imaging

register or log in to subscribe [?]
Article

How to Maintain Live Cells on a Microscope Stage

Are you preparing to set up live cell imaging experiments? You’ve got all your cell lines, antibodies, reagents, and protocol ready. You just want to wake up in the morning and enter into that dark room....
Read On
Article

How to Quantify Images in an Unbiased Way

Microscopists like to say that seeing is believing. The so-called belief in this context is a product of highly experienced imagination, which raises a concern about the objectivity. To remain objective, we try...
Read On
Sponsored Education

Controlling Color Image Quality in Microscopy: Start at the Beginning

The only constant with microscopy imaging is variability in both color and image quality. You only need to look at images in journal articles, posters, around your laboratory, or compare your images with a...
Read On
Article

Quick and Easy Automatic Cell Counting

Are you wondering how on earth you’re going to count thousands of cells across a stack of images? Well, I’m going to show you a simple method for automatic cell counting with ImageJ. For those of you...
Read On
Sponsored Education

Three-Dimensional Scanning Electron Microscopy for Biology

Scanning electron microscopy (SEM) is a powerful technique, traditionally used for imaging the surface of cells, tissues and whole multicellular organisms (see An Introduction to Electron Microscopy for...
Read On
Webinar

Color Image Quality Control in Microscopy

In this webinar, you will learn about color reproduction in microscopy images—and how to fix it if your color reproduction goes awry. The main points we will cover are: The reasons that could color...
View Webinar
Article

Polymers as Secondary Antibodies for Immunohistochemistry

Have you ever thought about using polymers for your immunohistochemistry experiments? Take this simple quiz and find out if polymers may be right for you: I order biotinylated secondary antibodies for...
Read On
Article

Lasers for Confocal Microscopy

Lasers were once called “a solution looking for a problem.” The word—which is an acronym for Light Amplification by Stimulated Emission of Radiation—used to conjure up images of deadly weapons from...
Read On
Article

A Biologist’s Guide to Choosing Your Fluorophore Palette

You may notice that nature is full of vibrant, even fluorescent, colors. The human eye detects wavelengths ranging from 390-700 nm and our perception of colors is actually a narrow part of the light spectrum....
Read On
Article

A Simple Method for Measuring Intracellular Fluorescence

Fortunately for microscopy users, measuring intracellular fluorescence has been made relatively simple through an ImageJ plugin called the Cell Magic Wand. For those of you unfamiliar with ImageJ, it’s a...
Read On