Techniques / DNA / RNA Manipulation and Analysis

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Choosing a Competent E.coli Strain

Of all the of competent E. coli cell strains available, which one should you choose? The choice of strain to use in a given experiment is determined in large part by the nature of the experiment and the set of...
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The World’s Fastest Miniprep?

I love this great time-lapse video entitled "a day in the lab". If only minipreps were this fast and easy! [youtube:http://www.youtube.com/watch?v=XKF_bItwkjk]
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Competent E.coli: To buy or not to buy?

Buying competent cells from commercial suppliers is convenient, provides a guarantee of quality and gives access to strains with a variety of in-built traits that assist with things like maintenance of...
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One Tube PCR Cloning Method

I love shortcuts, and this one is very good. Chun-Ming Liu of Plant Research International has a number of molecular biology protocols on his website,  but my favourite is his One Tube PCR Cloning Method. The...
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The Dark Side of Gene Synthesis

After writing my recent article on custom gene synthesis, I came across this article in the excellent Seven Stones systems biology blog that highlights the potential dark side of this emerging technology. The...
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Easier Gene Cloning With Positive Selection Cloning Vectors

Isn't it a pain digesting, purifying and dephosphorylating your cloning vector prep to eliminate prevent high background in your ligation/transformation? A new generation of positive selection cloning vectors...
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Custom Gene Synthesis: A PCR alternative.

The past few years have seen the emergence of custom gene synthesis as a useful but expensive service available for molecular biologists. Recently, the number of companies offering custom gene synthesis has...
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DNA replication, transcription and translation in action

While browsing you tube I came across this really nice animation of DNA replication, transcription and translation complete with imaginative sound effects. I hope you enjoy it as much as I did. ...
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Low cost DNA gel documentation

Equipment for photographing DNA gels stained with ethidium bromide (or other fluorescent dyes), doesn't have to cost thousands of dollars. These days, great pictures can be obtained with a standard digital...
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Plasmid Cut & Paste with Quikchange

I recently had a problem where I needed to cut out an expression cassette (a promoter coupled to a coding sequence) from one plasmid vector and paste it into another containing an expression cassette so I...
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