Techniques / Cloning & Expression

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Article

Benefits of the GUS Gene Reporter System in Plants

Gene reporters enable valuable insight into gene expression. The GUS gene reporter system is one of the popular and common plant reporter systems. GUS,  is short for glucuronidase, an enzyme in the bacterium...
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Article

Optimize Bacterial Protein Expression by Considering these 4 Variables

So, you have successfully cloned your gene of interest and are eager to purify buckets of protein. No matter your eventual application—kinetic experiments using a SPR instrument, structural analysis using...
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Article

Cut My Gene into Pieces– Introduction to Restriction Enzyme Cloning

At the heart of cloning are restriction enzymes. Restriction enzymes are a common tool in any molecular biology lab. Need to know how large your plasmid is? Cut it with a restriction enzyme. Need to chop your...
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Article

2015 Staff Picks: Cloning and Expression

We’re in the week between the 25th and 1st—the holiday week. If you’re in a college town, traffic is easy and parking restrictions have been lifted. With the staff picks of articles from the Cloning and...
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