Cloning and Expression
Improving the Expression of Insoluble and Inactive Proteins Using Osmotic Shock
Discover a better way to improve the expression of your insoluble and inactive proteins using osmotic shock.
Read MoreUnnatural Amino Acids: Essential Tools in Synthetic Biology and Your Research
Discover what unnatural amino acids are, their applications, and how they can be used in your research in our beginner’s guide.
Read MoreSelection Pressures: Alternatives to Antibiotics in Molecular Cloning
Want to reduce the use of antibiotics in the lab? Start with switching to alternative cloning methods that use alternative selection pressures.
Read MoreOptimize Bacterial Protein Expression by Considering these 4 Variables
So, you have successfully cloned your gene of interest and are eager to purify buckets of protein. No matter your eventual application—kinetic experiments using a SPR instrument, structural analysis using X-ray crystallography, or any other experiment—you’ll need to express your protein first. Now, it’s time to put your expression plasmid into E. coli and get…
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