Techniques / Cloning & Expression

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Article

Optimize Bacterial Protein Expression by Considering these 4 Variables

So, you have successfully cloned your gene of interest and are eager to purify buckets of protein. No matter your eventual application—kinetic experiments using a SPR instrument, structural analysis using...
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Article

Cut My Gene into Pieces– Introduction to Restriction Enzyme Cloning

At the heart of cloning are restriction enzymes. Restriction enzymes are a common tool in any molecular biology lab. Need to know how large your plasmid is? Cut it with a restriction enzyme. Need to chop your...
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Article

2015 Staff Picks: Cloning and Expression

We’re in the week between the 25th and 1st—the holiday week. If you’re in a college town, traffic is easy and parking restrictions have been lifted. With the staff picks of articles from the Cloning and...
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