Techniques / Cells and Model Organisms

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World of Microbes Part 4: Food and Biofuels.

In this World of Microbes series, so far we’ve covered the importance of microbes in medicine including their use in vaccines, antibiotics, probiotics and protein production of antibodies for immunotherapy....
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Mastering the Art of Growing THP-1 cells

Tissue culture can sometimes seem like a black art. Too careful—your cells go down. Not careful enough—your cells go down. A butterfly flutters its wings in the middle of the Atlantic Ocean—your cells go...
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Article

Chromium Release Assay: The Old School Way of Testing Cytotoxic T Cells

There are several methods you can use to see if your T cells are cytotoxic, but a chromium release assay using radioactive 51chromium (51Cr) is one of the oldest. It gives good results, and is great for labs...
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Article

Pre-Analytical Sample Handling: What Can You Do Better?

If you study human disease, you will likely handle a pre-analytical sample or two (or hundreds).  For example, you could handle whole blood, serum or plasma, tissue biopsies, urine, fecal samples,...
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Article

Three Steps for Setting up a Drug Screening Assay

An anti-cancer drug or antibody drug conjugate (ADC) screening assay is the first step to establish the utility of a drug candidate in killing cancer cells. Nevertheless, these assays are time consuming and...
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Introducing You to the Wonderful World of Microbes!

Welcome to the microbe series where we have a very exciting line-up planned over the coming months. Here we will talk about everything microbial, including the uses of microbes in industry and medicine,...
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Four Tips for Working with Human Clinical Samples

While using human clinical samples in your research can provide robust and heterogeneous results applicable to larger portions of the population, working with these samples presents its own set of...
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AAV Production Part I: Tips for Setting Up

What is Adeno-Associated Virus? Adeno-associated virus (AAV) is a popular gene therapy vector. Different AAV serotypes infect different cell and tissue types with varying efficiency, so it’s a good idea to...
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Article

How to Overcome Minor Issues in Anchorage-Independent Assays

Anchorage-independent assays test the ability of cells to grow independent of a solid surface. The assay is used to check the malignant potential of cancer cells. Cancer researchers generally do this...
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Article

Phagebiotics: Part 1

The enemy of my enemy is my friend –Ancient Sanskrit proverb Luna, 20 July 1969. Neil Armstrong set his foot in another world for the first and only time in human history. But this is not a story about...
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