Techniques / Analytical Chemistry and Chromatography Techniques

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Under Pressure: Tips for Keeping Your HPLC Up and Running Properly

If you’re anything like me, your biggest lab fear is working with expensive equipment prone to damage. HPLC is a wonderful tool, capable of separating, identifying, and quantifying a vast array of compounds,...
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Article

Using a Gel Filtration Chromatogram to Estimate Molecular Weight

Gel filtration chromatography (also known as size exclusion chromatography, molecular sieve chromatography, or gel permeation chromatography) is based on the differential distribution of the components in a...
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Article

FTIR Spectroscopy: Every Window Needs a Good Acid Wash

Fourier Transform Infrared spectroscopy (FTIR spectroscopy) is a useful and exquisitely sensitive technique used to identify and quantify unknown compounds, as well as study fine molecular details. However,...
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Gel Filtration Chromatography Media: What You Need to Know

Do you use pre-packed Superdex 75 or Superdex 200 columns for gel filtration chromatography? Ever wonder if other media could better address your purification needs? Look no further. Your quest ends...
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How Separation Works in Column Chromatography Methods

Separation in column chromatography relies on differences. Molecules vary in size, charge, polarity, and solubility. We leverage these differences to distribute molecules between a stationary phase and a mobile...
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Fine-Tune Your MALDI-TOF to Produce Good-looking Mass Spectra

Mass Spec is all about getting the perfect peaks. Without a good peak assigning the correct mass is impossible and you cannot make accurate identifications. Make sure you know how to adjust your MALDI-TOF...
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Article

The Basics of Running a Chromatography Column

Column chromatography is a common technique used to separate individual compounds from a mixture. You can use column chromatography on both a small or large scale to isolate and purify material for use on a...
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Troubleshooting Thin Layer Chromatography: Some TLC for Your TLC

The whole TLC technique sounds easy to do, but it can be difficult and tricky during interpretation or give unexpected results, especially when working with biomolecules. For this reason, it is important to be...
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Article

Thin Layer Chromatography: The Basics

Like most other chromatographic techniques, thin layer chromatography (TLC) separates out individual compounds from a mixture depending upon the polarity of each compound. The solvent system travels up a silica...
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Article

The Many Uses of Thin Layer Chromatography

While working as biologists, we often come across mixtures of compounds, and the first question that strikes our minds is ‘what are the components in this mixture?’ One might think of using chemical assays...
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