Techniques / Analytical Chemistry and Chromatography Techniques

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FTIR Spectroscopy: Every Window Needs a Good Acid Wash

Fourier Transform Infrared spectroscopy (FTIR spectroscopy) is a useful and exquisitely sensitive technique used to identify and quantify unknown compounds, as well as study fine molecular details. However,...
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Article

Gel Filtration Chromatography Media: What You Need to Know

Do you use pre-packed Superdex 75 or Superdex 200 columns for gel filtration chromatography? Ever wonder if other media could better address your purification needs? Look no further. Your quest ends...
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Article

How Separation Works in Column Chromatography Methods

Separation in column chromatography relies on differences. Molecules vary in size, charge, polarity, and solubility. We leverage these differences to distribute molecules between a stationary phase and a mobile...
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Article

Fine-Tune Your MALDI-TOF to Produce Good-looking Mass Spectra

Mass Spec is all about getting the perfect peaks. Without a good peak assigning the correct mass is impossible and you cannot make accurate identifications. Make sure you know how to adjust your MALDI-TOF...
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Article

The Basics of Running a Chromatography Column

Column chromatography is a common technique used to separate individual compounds from a mixture. You can use column chromatography on both a small or large scale to isolate and purify material for use on a...
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Article

Troubleshooting Thin Layer Chromatography: Some TLC for Your TLC

The whole TLC technique sounds easy to do, but it can be difficult and tricky during interpretation or give unexpected results, especially when working with biomolecules. For this reason, it is important to be...
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Article

The Many Uses of Thin Layer Chromatography

While working as biologists, we often come across mixtures of compounds, and the first question that strikes our minds is ‘what are the components in this mixture?’ One might think of using chemical assays...
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Article

Carrying You Through Gas Chromatography

Maybe you found a paper in which they used gas chromatography (GC) to analyze a sample that is similar to yours and now you are wondering if you should try it too. If you are hesitant because you know little...
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Article

How to Choose Your MALDI (Soul) Matrix

gif by Kronin Okay not talking about that kind of matrix. But the kind of matrix that is crucial for MALDI (Matrix-Assisted Laser Desorption Ionization). I talked briefly about MALDI in my last article,...
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Article

How to Measure Molecular Weight with Gel Permeation Chromatography (GPC)

So you have a new polymer, unknown protein, or material combination but don’t know its molecular weight? An easy way to measure molecular weight of your sample is gel permeation chromatography (GPC). GPC is...
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