Techniques / Analytical Chemistry and Chromatography Techniques

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The Importance of Metabolomics Research

Metabolomics may sound like a fictional character in the famous comic series “Asterix”, but it is very important in understanding systems biology and in clinical research against various...
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Critical Factors for Full Characterization of Biosimilars

Today, about half of all therapeutic drug approvals by the US Food and Drug Administration (FDA) are for biological drugs. That number is expected to rise to 75 percent by 2025. When these biologics come off...
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Key Analytical Challenges for Antibody Drug Conjugates

Currently, there are more than 75 antibody drug conjugates (ADCs) in various stages of pre-clinical and clinical development. The combination of a targeted antibody coupled with a cytotoxic small-molecule drug...
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Article

Pack a Chromatography Column Like a Pro

You already learned the basics of column packing. When moving to more automated system using low pressure liquid chromatography systems, you can use pre-packed columns. But in order to compare several resins in...
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To Run or to Fly: A Comparison Between HPLC and GC

Many chemical compounds, including drugs and metabolites, can be analyzed by either gas chromatography (GC) or high-performance liquid chromatography (HPLC). Because both techniques function under the same...
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Ten Ways to Give Your Surface Plasmon Resonance Experiments a Hand

Surface Plasmon Resonance (SPR) is the gold standard for measuring biomolecular binding without the need for labeling (i.e., label free detection of kinetics). SPR is especially valuable because it doesn’t...
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Article

How to Clean and Unclog Your HPLC Column

In my last article, I discussed how to best keep your lab’s HPLC running smoothly. However, even the best-maintained HPLCs and columns need periodic cleaning. Today, I’ll describe how to identify and...
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Article

How to Separate Nucleotides Using Ion-paired Reverse Phase HPLC

If you work in the field of molecular biology, there is hardly a day that goes by that you don’t use nucleotides. But beyond the use of the four well-known deoxynucleotides in PCR, you can use nucleotides for...
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Article

Under Pressure: Tips for Keeping Your HPLC Up and Running Properly

If you’re anything like me, your biggest lab fear is working with expensive equipment prone to damage. HPLC is a wonderful tool, capable of separating, identifying, and quantifying a vast array of compounds,...
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Article

Using a Gel Filtration Chromatogram to Estimate Molecular Weight

Gel filtration chromatography (also known as size exclusion chromatography, molecular sieve chromatography, or gel permeation chromatography) is based on the differential distribution of the components in a...
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