Techniques

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Development of a Potential Recombinant Protein Vaccine in E. Coli

Recombinant proteins can be powerful stimulators of the immune system making them good candidates for vaccines. Using the intracellular parasite, Leishmania donovani as an example, this webinar will take you through the steps required for development and testing of a recombinant vaccine. In this talk you will learn how to: Express and purify protein from […]

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In GenScript, Protein Expression & Analysis 3rd of May, 2017 Presented by: C Patrick McAtee
Webinar

Guide to CRISPR/Cas9 Delivery: How to Maximize Your Editing Efficiency

In this webinar, you will learn how to maximize your genome editing efficiency using CRISPR/Cas9 and how to apply this technique in your research. The main points in the webinar will include: How to...
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Article

Fluorescence Microscopy – the Magic of Fluorophores and Filters

What Is Fluorescence Microscopy All About? Maybe you are sitting in front of your computer wondering whether fluorescence microscopy might be the technique to help you light the bulb on your project. Or you...
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Article

Demystifying NGS: Depth Coverage and Deep Sequencing

NGS is not a three-headed monster. However, it can be a difficult concept to grasp—especially when you are getting started.  There is a lot of new terminology, and a whole new world to discover: both in the...
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How to Choose Quality Antibodies for Successful Western Blotting

Successful western blotting means achieving unambiguous results, and this requires a sensitive and specific antibody-antigen interaction. Consequently, high quality antibodies are critical for reliable and...
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Article

Top 5 of the most commonly used cell lines!

Cell lines are an invaluable scientific tool. They allow us to dissect the internal workings of tissues in a controlled environment without the ethical implications of working with whole organisms. Starting...
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Article

How to Store Your Reagents, so They ‘Do Exactly What It Says on the Tin’

Your reagents should do ‘Exactly what they say on the tin.’  This only happens though if you look after them in the way the manufacturer states on their data sheets. We have all been guilty of using...
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Article

Fluorescent Western Blotting: Lowdown and Advantages

In this article, I will introduce you to the world of fluorescent western blotting. Firstly, we'll find out how it compares to chemiluminescent western blotting. Then, we will learn how infrared fluorescent...
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Article

Setting up a Fermentation or Perpetuum Mobile Cell Culture

Some names are confusing. For example, ant-lion is not an ant - or a lion. Likewise, fermentation in the scientific sense does not involve using a ferment or brewing beer. In science, fermentation is the...
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Article

Quantifying & Assessing RNA: TE or not TE?

Red Pill or Blue? Carrying out science often involves many difficult decisions! I see it all the time in RNA protocols - the “gracious” option of using purified water or Tris-EDTA (TE) buffer to dissolve...
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Article

Divide and Conquer: How to Setup Your First Droplet Digital PCR Experiment

Droplet digital PCR?  It's easy. Because we’re here to guide you through it. We recently introduced you to the principles of digital PCR technology and how it differs from qPCR. In a nutshell, digital PCR...
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