Seeing is Believing: An Introduction to Imaging Flow Cytometry

What if there was a way to take the power and speed of a flow cytometer and couple it with the resolution of a microscope? Imaging flow cytometry does just that! Flow cytometry is […]

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Better Plasmid Midipreps Part II: What Causes Low Yields?

Recently we received a question from Bitesize Bio reader Sonia after our article How to: Get Better Plasmid Midiprep Yields. She asked: "What could be the problem when one sample gives a good yield while […]

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How to identify supercoils, nicks and circles in plasmid preps

One of my favorite things to do with a student the first time they work with plasmid DNA is to have them run an agarose gel containing 2 samples:  uncut plasmid DNA, and plasmid […]

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How to: Get Better Plasmid midiprep Yields

I get many people complaining to me about poor DNA yields from commercial plasmid plasmid prep kits. In this article I will explain the main pitfalls in plasmid isolation and how to avoid them. […]

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Why You Should Talk to Sales Reps

People often joke about hiding from sales reps, but the fact of the matter is that valuable opportunities are lost when scientists disappear into thin air. Think twice before attempting your own vanishing act; […]

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How to Switch Mentors, Part 1: Recognizing Red Flags

Grad school is a long, hard, long, time-consuming, and–wait for it–long process. A bad relationship with your primary mentor can make it worse, and may even drive you away from a science career.  Unfortunately, […]

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Keeping 'em Alive! Incubators and Chambers for Live-Cell Imaging

Live-cell imaging is a powerful technique that allows you to image dynamic cell processes, such as protein trafficking, signal transduction, endocytosis, and exocytosis, etc. However, to perform your own live-cell imaging you need the […]

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Equilibrating your way to a perfect Western blot

If you are struggling to optimise your Western blot protocol, one step to consider is the equilibration of your gel and membrane before transfer. Wondering what this step achieves and whether it’s necessary? You’re […]

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Getting in the Flow: Online Resources for Flow Cytometry

You are sitting in a seminar when you glimpse it:  the figure that beats all other figures -  a beautiful contour plot – and you realise that a similar figure is what you need […]

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10 Favorite Online Tools for Molecular Biology

What did we do before the internet? And where would we be without handy online molecular biology tools? Apparently in the ‘olden days’ doing a simple gene or protein alignment required programs that used […]

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