Don’t Be Another P-value Victim

In previous articles, I’ve primed you on hypothesis testing and how we are forced to choose between minimising either Type I or Type II errors. In the world of the null hypothesis fetish, the […]

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How to Extract High-Quality RNA for Microarray Analysis

Microarrays are one of the most in-depth ways of determining cellular gene expression levels of thousands of genes simultaneously.  They are able to help determine: Gene function and cellular processes Gene regulation and  interactions […]

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Mind Your P’s And Q’s: A Short Primer On Proofreading Polymerases

For applications such as site-directed mutagenesis, it is often recommended that you use a proofreading polymerase (also known as high-fidelity polymerases) to minimize the risk of introducing unintended point mutations.  But what is a […]

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How Text-Mining Tools Can Improve Your Literature Searches

Before starting any new research project, it’s essential that you have as complete an understanding as possible of the current research literature. Knowing what other people have done will prevent you from duplicating existing […]

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Are Purified Primers Really Necessary For Site-Directed Mutagenesis?

Most site-directed mutagenesis protocols strongly recommend that you use only PAGE- or HPLC-purified primers to mutate plasmid templates.  Using purified primers is supposed to minimize the introduction of unintended mutations, thus drastically improving the […]

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When Silence Speaks Volumes: Using RNAi to Investigate Gene Function

RNA interference (RNAi) may have originated as a defense mechanism to protect cells against foreign genes introduced by viruses. This concept has since been put to use to create a powerful experimental tool for […]

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5 Ways to Improve Your ELISAs

ELISAs are often used for detecting and quantifying substances such as peptides, proteins, antibodies and hormones in research and diagnostics. While it has become easier to perform ELISAs, thanks to the various commercially available […]

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What Do You Get For The Grad Student Who Has Everything?

As the holiday season approaches, I'm sure many of you are asking yourself what to get for that special grad student in your life.  Or perhaps you'd like to treat yourself this year!  But […]

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How to Land a PostDoc Position

You have been toiling away at your thesis project for years and you think the end is in sight. Now the big question is “What’s next?” If you think you might want to move […]

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Making a List, Checking it Twice: 5 End-of-the-year Lab Tasks

A lot of effort is spent on running experiments…and occasionally it can feel like an almost equal amount of effort is spent on administrative tasks! Policy compliance is important for keeping everyone in the […]

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