3 Approaches to Western Blot Transfer

I think that transferring Western blots is one the most enjoyable tasks to do in a lab: it’s quick, it’s messy, and on some gleeful level, it feels like a child’s art project gone […]

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What Can NMR Do For You? – Part One

Having done my PhD work in an NMR lab, I sometimes feel like NMR doesn’t get the credit it deserves. As a structural tool, it always seems to play second fiddle to crystallography, and […]

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4 Great Websites for Bench Scientists

Friends and colleagues are excellent sources of scientific information. The Internet is too, because it is a virtual library at your fingertips. If you are looking for a great solution to a common problem […]

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How Do YOU Make Sure That Your Western Blots are Evenly Loaded?

For Western blot data to be reliable, it is important that you load known amounts of sample into each lane of the gel.  This is of particular importance if you are doing a quantitative […]

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How To Start Your PhD The Right Way

You have just finished your undergraduate studies, or perhaps you have been working in the field for a while, you are full of energy and zest and you want to prove to the world […]

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Doesn’t Play Well with Others- The Chemistry of the Autoclave

While Luria-Bertani broth (LB) has long been the fuel that powered Molecular Biology and Biochemistry, there is an increasing movement towards more specialized and complex bacterial media formulations such as Terrific Broth (TB), Plasmid […]

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How To Analyze Your Single Nucleotide Polymorphism (SNP) Chip Data

So, you’re sitting there with your list of significant SNPs, thinking, “what do I do now”?  Hopefully this article can point you in the right direction! So far, you will have extracted genomic DNA […]

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The Poor Man’s Polariser…Got Shades?

I recently introduced you to the concept of polarising microscopy. Naturally, if evaluating refractile material is an everyday part of your research, it is definitely worth investing in a professional polariser modification for your […]

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An Introduction to Tandem Affinity Purification

Tandem affinity purification is a development of existing techniques for purifying protein complexes from cells in physiological conditions. It was first described over ten years ago and has become a commonplace laboratory tool. In […]

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The Perfect Slice: Preparing Tissue Samples For IHC

When you stop to think about it, tissue slices for immunohistochemistry (IHC) undergo quite a lot of handling. From chemical reactions to washes – even manipulations and transfers between baskets and microtubes – final […]

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