An introduction to RNA-seq

RNA sequencing (Wang 2009) is rapidly replacing gene expression microarrays in many labs. mRNA (and other RNA’s) are converted to cDNA that is used as the input to a next-generation sequencing library preparation. RNA-seq […]

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!BAM! !POW!: How to Become a Better Job Search Street Fighter – Part One

Career searches and job hunts are painful: answering ads, resumé tweaking, phone calls, interviews, preparation, sweat, tears, fear of rejection, etc. But it’s funny, even after investing all of this effort, the same mistakes […]

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The Invisible Horde: Attacking Mycoplasma Infections

Mycoplasma infections are very, very bad news; these special prokaryotes can rapidly spread through your cell culture and inhibit cell proliferation, induce apoptosis, cytokines and radicals, and otherwise transform your cells. Worst of all, […]

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How to Care for Your pH Meter

Is there anything more tedious than pH-ing a solution? Standing there adjusting the pH of your buffer, adding acid or alkali drop by drop until you get to the right pH… With pH-ing being […]

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Top Tips For Peaceful Lab Communities

Picture the scene: standing at your bench, you look up at the shelf of neatly-labelled bottles of fresh buffers, you see your pipettes arrayed in size order by your right hand, the gleaming expanse […]

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How to Keep Track of Lab Orders

How often have you torn apart the lab looking for the reagent you need right now for some thawing samples?  That reagent which you (possibly) ordered a week ago and which (maybe) came in […]

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How to to Speed up Commercial Midi and Maxi Plasmid Preps

Commercial kits for isolation of large quantities of plasmid DNA generally rely on standard alkaline lysis followed by an affinity chromatography column-based method to purify DNA. Compared to traditional cesium chloride banding or PEG […]

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Wasting Antibodies Doesn’t Float Your Boat? Try Floating Your Blot Instead!

Western blots may be great for visualizing protein expression, but they can be a perfect way to waste your precious antibody stocks if you follow the normal protocol. Thankfully, you don’t have to follow […]

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Is Your Science Making an Impact?

It’s pretty likely you’ll have heard of impact factors, either through colleagues talking about them in the lab, or from a journal homepage advertising its latest score. Whilst impact factor is a relatively artificial […]

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How to Find a Postdoc Overseas

I have always had it in the back of my mind that I would like to live and work abroad for a year or two. This was augmented in my mind by the mainstream […]

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