Pseudoreplication: Don’t Fall For This Simple Statistical Mistake

Now we come to the third part of our trifecta; in the last two posts I have gone over p-values and how they determine significance in null hypothesis testing, and we talked about degrees […]

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Build A CV You Can Be Proud Of – Part III: Analytical Skills… Including the Dreaded Statistics!

In the previous article in this series, we covered teamwork and networking. Now it’s time to move on to what many people consider the most boring part of the lab work: the analysis. I […]

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How Free is Your Degree?

In the last post I talked about p-values and how we define significance in null hypothesis testing. P-values are inherently linked to degrees of freedom; a lack of knowledge about degrees of freedom invariably […]

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Don’t Be Another P-value Victim

In previous articles, I’ve primed you on hypothesis testing and how we are forced to choose between minimising either Type I or Type II errors. In the world of the null hypothesis fetish, the […]

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How to Extract High-Quality RNA for Microarray Analysis

Microarrays are one of the most in-depth ways of determining cellular gene expression levels of thousands of genes simultaneously.  They are able to help determine: Gene function and cellular processes Gene regulation and  interactions […]

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Mind Your P’s And Q’s: A Short Primer On Proofreading Polymerases

For applications such as site-directed mutagenesis, it is often recommended that you use a proofreading polymerase (also known as high-fidelity polymerases) to minimize the risk of introducing unintended point mutations.  But what is a […]

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How Text-Mining Tools Can Improve Your Literature Searches

Before starting any new research project, it’s essential that you have as complete an understanding as possible of the current research literature. Knowing what other people have done will prevent you from duplicating existing […]

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Are Purified Primers Really Necessary For Site-Directed Mutagenesis?

Most site-directed mutagenesis protocols strongly recommend that you use only PAGE- or HPLC-purified primers to mutate plasmid templates.  Using purified primers is supposed to minimize the introduction of unintended mutations, thus drastically improving the […]

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When Silence Speaks Volumes: Using RNAi to Investigate Gene Function

RNA interference (RNAi) may have originated as a defense mechanism to protect cells against foreign genes introduced by viruses. This concept has since been put to use to create a powerful experimental tool for […]

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5 Ways to Improve Your ELISAs

ELISAs are often used for detecting and quantifying substances such as peptides, proteins, antibodies and hormones in research and diagnostics. While it has become easier to perform ELISAs, thanks to the various commercially available […]

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