How Free is Your Degree?

In the last post I talked about p-values and how we define significance in null hypothesis testing. P-values are inherently linked to degrees of freedom; a lack of knowledge about degrees of freedom invariably […]

view article

Don’t Be Another P-value Victim

In previous articles, I’ve primed you on hypothesis testing and how we are forced to choose between minimising either Type I or Type II errors. In the world of the null hypothesis fetish, the […]

view article

How to Extract High-Quality RNA for Microarray Analysis

Microarrays are one of the most in-depth ways of determining cellular gene expression levels of thousands of genes simultaneously.  They are able to help determine: Gene function and cellular processes Gene regulation and  interactions […]

view article

Mind Your P’s And Q’s: A Short Primer On Proofreading Polymerases

For applications such as site-directed mutagenesis, it is often recommended that you use a proofreading polymerase (also known as high-fidelity polymerases) to minimize the risk of introducing unintended point mutations.  But what is a […]

view article

How Text-Mining Tools Can Improve Your Literature Searches

Before starting any new research project, it’s essential that you have as complete an understanding as possible of the current research literature. Knowing what other people have done will prevent you from duplicating existing […]

view article

Are Purified Primers Really Necessary For Site-Directed Mutagenesis?

Most site-directed mutagenesis protocols strongly recommend that you use only PAGE- or HPLC-purified primers to mutate plasmid templates.  Using purified primers is supposed to minimize the introduction of unintended mutations, thus drastically improving the […]

view article

When Silence Speaks Volumes: Using RNAi to Investigate Gene Function

RNA interference (RNAi) may have originated as a defense mechanism to protect cells against foreign genes introduced by viruses. This concept has since been put to use to create a powerful experimental tool for […]

view article

5 Ways to Improve Your ELISAs

ELISAs are often used for detecting and quantifying substances such as peptides, proteins, antibodies and hormones in research and diagnostics. While it has become easier to perform ELISAs, thanks to the various commercially available […]

view article

What Do You Get For The Grad Student Who Has Everything?

As the holiday season approaches, I'm sure many of you are asking yourself what to get for that special grad student in your life.  Or perhaps you'd like to treat yourself this year!  But […]

view article

How to Land a PostDoc Position

You have been toiling away at your thesis project for years and you think the end is in sight. Now the big question is “What’s next?” If you think you might want to move […]

view article
more articles

Share your tips, advice & wisdom on Bitesize Bio

We gather the best tips, advice and wisdom from you guys at the bench and publish them to help each other improve in the lab.

What could you add to this collection? Click here to explore how you could contribute to Bitesize Bio.