Overlap Extension PCR Cloning

If efficient cloning is what you are after, you must give Overlap Extension PCR Cloning a go! This restriction enzyme and T4 DNA ligase-free technique is faster, more reliable and easier to troubleshoot than […]

view article

The Who’s Who of Super Resolution Microscopy, Part 1

The 'diffraction limit' of a microscope is the minimum distance between two fluorophores where they can be still be discriminated as two separate objects. This diffraction limit has long constrained attempts by biologists to […]

view article

Sequencing the microbiome: re-discovering our invisible allies

Scientific breakthroughs have profound effects on how we view and treat the world that surrounds us. Especially in the microscopic level, scientific discoveries provide the only understanding we have of Nature. One such case […]

view article

One Fish, Two Fish, Red Fish, Blue Fish: Are You Using the Right Scale?

There are many things that vary from one scale to the next. When picking which scale to buy or use, there are a few things to take into account: the two biggies being the […]

view article

Mischief in the Mastermix: Bothersome Bubbles

It happens to the best of us. You’re minding your own business and suddenly out of nowhere your mastermix is a bubble bath and your primers are enjoying a froth party. Let’s talk about […]

view article

Crap in, crap out: Flushing Out The Problems in Your Flow Cytometry Data

“What Have You Done To My Cells??!!!” This cry of pain from researchers, frequently aimed at core facility operators, is heard after receiving incomprehensible data for an invaluable tube of cells. Equally baffling to […]

view article

Stand By Your Poster

A lot of scientists hate presenting posters. Because more often than not, you stand near your carefully designed, full of great results placard, all alone, while there is a crowd gathered two posters down. […]

view article

MIQE Guidelines for Digital PCR

MIQE what's that? When writing dPCR materials and methods for a paper have you ever pondered what information you should include? This is where the MIQE guidelines will really help. Guidelines for minimum information […]

view article

Starters and Finishers: Who are the Most Valuable in the Lab?

Life seems to smile often on the work of the Starters. They pitch one idea after another and get to claim all the credit, whilst depending on others – namely the Finishers – to […]

view article

All for one and one for all – Fluorescence Minus One Controls

Ever done a multicolour flow cytometry experiment, run all your controls, done your compensation and then started to analyse your data and realised that you can’t work out where to put your gates? To […]

view article
« Previous PageNext Page »

Article Categories